StraightFrom® Buffy Coat MicroBeads

Cell isolation directly from buffy coat 

  • Highly pure cells straight from buffy coat
  • No density gradient centrifugation, no erythrocyte lysis
  • Easy and fast protocol with only a few handling steps
StraightFrom MicroBeads allow magnetic cell separation directly from buffy coat. Leukocyte subsets can be purified using either the autoMACS® Pro Separator, the MultiMACS™ Cell24 Separator Plus or manual separators. Pre-enrichment of white blood cells, e.g. by density gradient centrifugation, is not required. The purified cells are well-suited for further flow cytometric analyses, molecular biology applications, and functional studies.

Automated cell isolation with the MultiMACS Cell24 Separator Plus

Automated cell isolation with the MultiMACS™ Cell24 Separator Plus
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The MultiMACS Cell24 Separator Plus is ideally suited to process an entire buffy coat in a single run with the StraightFrom Buffy Coat MicroBeads. The 30-minute isolation procedure results in cell populations with high purities and recovery rates. With 12 Whole Blood Columns (included in the kit), cell isolation directly from buffy coats becomes as easy as it gets. The instrument allows for time saving when processing large sample volumes, while providing reliability and reproducibility in day-to-day cell separation. 

Manual cell isolation 

Manual cell isolation together with Whole Blood Columns
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StraightFrom Buffy Coat MicroBead Kits are suited for the separation of cell populations directly from an entire or partial buffy coat. Together with Whole Blood Columns (included in the kit) and magnetic separators such as the QuadroMACS™ Separator, the manual method allows flexible handling of samples of different sizes and donors. 

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