CD8 MultiSort Kit, human

CD8 MultiSort Kit, human

The CD8 MultiSort Kit is a direct magnetic labeling system designed for the isolation of distinct CD8
+
T and NK cell subsets from human PBMCs, bone marrow, cell cultures, or single-cell suspensions from tissue.

Detailed separation procedure

The CD8
+
cells are isolated with CD8 MultiSort MicroBeads. Then the magnetic particles are enzymatically released. The selected CD8
+
cells can again be magnetically labeled and sorted for a second antigen.

Downstream applications

The CD8 MultiSort Kit can be used to isolate specific CD8
+
T cell or NK cell subsets. The selected CD8
+
cell population can be sorted according to a second marker of interest, e.g., CD45RA, CD45RO, CD16, CD25
1
, CD27
2
, CD28, CD30, CD56, CD62L, and CD69. The CD8 MultiSort Kit has also been used for the isolation of IL-4–secreting CD8
+
T cells in combination with the IL-4 Secretion Assay – Cell Enrichment and Detection Kit.
1

Columns

MS, LS, XS, or autoMACS
®
Columns.
  • Selected references

    1. Schwaiger, S. et al. (2003)
      IL-4-producing CD8
      +
      T cells with a CD62L
      ++(bright)
      phenotype accumulate in a subgroup of older adults and are associated with the maintenance of intact humoral immunity in old age.
      J. Immunol. 170: 613-619
    2. Sauce, D. et al. (2003)
      Retrovirus-mediated gene transfer in polyclonal T cells results in lower apoptosis and enhanced
      ex vivo
      cell expansion of CMV-reactive CD8 T cells as compared with EBV-reactive CD8 T cells.
      Blood 102: 1241-1248
  • Special protocols

Product options: 1
for 1×
10
9
total cells
EUR  664,00

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