CD16+ Monocyte Isolation Kit, human

CD16
+
Monocyte Isolation Kit
, human

The CD16
+
Monocyte Isolation Kit has been developed for a two-step isolation of the CD14
+
CD16
+
monocyte subset from PBMCs.

Background information

Intermediate and non-classical monocytes together are referred to as CD16
+
monocytes
1
and account for about 5–10% of all blood monocytes. CD14
+
CD16
+
monocytes differ from CD14
+
CD16
-
monocytes with regard to phenotype and immunological function. CD16
+
monocytes are, for example, considered more mature and are believed to have a higher T cell stimulatory capacity than CD16
-
monocytes.
2, 3

Detailed separation procedure

Isolation of CD16
+
monocytes is performed in a two-step procedure. Prior to separation, cells are incubated with FcR Blocking Reagent. Granulocytes and NK cells are then magnetically labeled with a cocktail of CD15 MicroBeads and CD56 MicroBeads, and depleted over a MACS Column. The flow-through in this first separation step contains pre-enriched, unlabeled CD16
+
monocytes. In the second separation step, CD16
+
monocytes are efficiently isolated by positive selection after labeling with CD16 MicroBeads.

Downstream applications

Isolated blood CD16
+
monocytes are suitable, for example, for investigations on CD16
+
monocyte maturation, migration, and differentiation, or for studies on antigen uptake, antigen processing and presentation to T cells.

Columns

For the first magnetic separation (depletion): LD or autoMACS
®
Columns. For the second magnetic separation (positive selection): MS, LS, or autoMACS Columns.
  • Selected references

    1. Ziegler-Heitbrock et al. (2010) Nomenclature of monocytes and dendritic cells in blood. Blood 116: e74-80
    2. Ziegler-Heitbrock, H. W. (1996)
      Heterogeneity of human blood monocytes: the CD14
      +
      CD16
      +
      subpopulation.
      Immunol. Today 17: 424-428
    3. Grage-Griebnow, E. et al. (2001) Heterogeneity of human peripheral blood monocyte subsets. J. Leukoc. Biol. 69: 11-20
Product options: 1
for 2×
10
9
total cells
EUR  778,00

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