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Memory B cells were isolated from mouse spleen using the Memory B Cell Isolation Kit, mouse. Cells were fluorescently stained with CD45R (B220)-VioBlue (#130-094-287), CD38-PE (#130-097-087) and Anti-IgM-FITC (#130-095-906) and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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