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Flp-mediated recombination in mouse ES cells:
HM1 mouse embryonic stem cells carrying a GFP reporter gene flanked by two FRT sites were electroporated with 15 µg StemMACS Flp Recombinase mRNA and analyzed by flow cytometry 96h after transfection. 88% of all cells were GFP-negative indicating successful Flp-mediated recombination.
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