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CD4+ T cells were isolated from human peripheral blood mononuclear cells (PBMCs). Cells were then stimulated with 50 ng/mL Phorbol 12-myristate 13-acetate (PMA) and 1 µg/ml Ionomycin for 6 hours and then positively selected for their IL-17A expression. After cultivation for 11 days, cells were restimulated with 50 ng/mL PMA and 1 µg/ml Ionomycin for 6 hours, followed by an incubation with 1 µg/ml Brefeldin A for 4 hours. Cells were then fixed, permeabilized and stained with the REA Control (I) antibodes (left image) or with Anti-IL-17F antibodies. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris were excluded from the analysis based on scatter signals.
Other clones | Overlap in epitope recognition with REA1012 |
---|---|
O33-782 | ++ |
SHLR17 | ++ |
LN2-9C4 | ++ |
Cells were incubated with an excess of purified unconjugated IL-17F (REA1012) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker. |
Clone | REA1012 |
---|---|
Clonality | monoclonal |
Isotype | recombinant human IgG1 |
Isotype control | REA Control Antibody (I), human IgG1 |
Host | human cell line |
Type of antibody | Primary antibodies, Recombinant antibodies |
Species | human |
Antigen | IL-17F |
Alternative names of antigen | Interleukin-17F, CANDF6, ML-1 |
Molecular mass of antigen [kDa] | 15 |
Distribution of antigen | granulocytes, mast cells, T cells, basophils |
Entrez Gene ID | 112744 |
RRID | AB_2727728, AB_2727799, AB_2727729, AB_2727800, AB_2727730, AB_2727801, AB_2727731, AB_2727798 |
Other clones | Overlap in epitope recognition with REA1012 |
---|---|
O33-782 | ++ |
SHLR17 | ++ |
LN2-9C4 | ++ |
Cells were incubated with an excess of purified unconjugated IL-17F (REA1012) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker. |
Clone | REA1012 |
---|---|
Clonality | monoclonal |
Isotype | recombinant human IgG1 |
Isotype control | REA Control Antibody (I), human IgG1 |
Host | human cell line |
Type of antibody | Primary antibodies, Recombinant antibodies |
Species | human |
Antigen | IL-17F |
Alternative names of antigen | Interleukin-17F, CANDF6, ML-1 |
Molecular mass of antigen [kDa] | 15 |
Distribution of antigen | granulocytes, mast cells, T cells, basophils |
Entrez Gene ID | 112744 |
RRID | AB_2727728, AB_2727799, AB_2727729, AB_2727800, AB_2727730, AB_2727801, AB_2727731, AB_2727798 |
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