Recombinant human IL-7 (interleukin 7) stimulates the development of lymphoid progenitors, T, and B cells. The hematopoietic cytokine is crucial for the proliferation of T and B cells, their survival, and homeostasis. Optimized for use in cell culture, functional assays, and differentiation studies.

Data and images for Human IL-7

Figures

Figure 1

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Human IL-7 activity assay.
The biological activity of Human IL-7, premium grade was determined by proliferation assay using mouse 2E8 cells. The assay was calibrated with the reference standard for human IL-7 (NIBSC 90/530) provided by the National Institute for Biological Standards and Control.

Figure 1

Human IL-7 activity assay.
The biological activity of Human IL-7, premium grade was determined by proliferation assay using mouse 2E8 cells. The assay was calibrated with the reference standard for human IL-7 (NIBSC 90/530) provided by the National Institute for Biological Standards and Control.

Figure 2

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SDS-PAGE of Human IL-7, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 2

SDS-PAGE of Human IL-7, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 3

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Human IL-7 biological activity.
Activity of Human IL-7, premium grade (red bar) was compared to another commercially available product (black bar).

Figure 3

Human IL-7 biological activity.
Activity of Human IL-7, premium grade (red bar) was compared to another commercially available product (black bar).

Figure 4

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Mass spectrometry analysis (ESI-MS) of Human IL-7, premium grade. The peak corresponds to the calculated molecular mass of 17382 Da.

Figure 4

Mass spectrometry analysis (ESI-MS) of Human IL-7, premium grade. The peak corresponds to the calculated molecular mass of 17382 Da.

Specifications for Human IL-7

Overview

Recombinant human IL-7 (interleukin 7) stimulates the development of lymphoid progenitors, T, and B cells. The hematopoietic cytokine is crucial for the proliferation of T and B cells, their survival, and homeostasis. Optimized for use in cell culture, functional assays, and differentiation studies.

Applications

Human IL-7 can be used for a variety of applications, including:
  • In vitro T cell expansion.
  • In vitro T cell priming.
  • In vitro differentiation of T cells and iNKT cells.
  • Investigation of IL-7 mediated signaling pathways.

Detailed product information

Background information

IL-7 is a member of the type I cytokine family. The primary sources of IL-7 are non-hematopoietic stromal cells in bone marrow, thymus, and lymphoid organs and tissues. It is a pleiotropic cytokine with central roles in modulating T cell development and peripheral T cell homeostasis. IL-7 can act both as a T cell growth factor as well as a critical anti-apoptotic survival factor for naive and memory T cells. IL-7 is related to IL-2 and signals through a heterodimeric receptor composed of the common cytokine signaling γ-chain and IL-7 receptor α-chain.

Biological activity

  • Proliferation of 2E8 cells (NIBSC 90/530)
  • research grade: ≥ 2×
    10
    7
    U/mg
  • premium grade: ≥ 5×
    10
    7
    U/mg
    (Typical specific activity: ≥ 3×
    10
    8
    U/mg
    )
  • We measure the biological activity of each batch of MACS Premium-Grade Cytokines and state the results in the Certificate of Analysis (CoA). Based on the lot-specific activity, exact doses of active cytokine can be applied to cell culture experiments. This allows for reproducible cell culture conditions without the need for time-consuming lot-to-lot testing.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Resources for Human IL-7

Documents and Protocols

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Human IL-7

Publications

  1. Shomuradova, A. S. et al. (2020) SARS-CoV-2 epitopes are recognized by a public and diverse repertoire of human T cell receptors. Immunity 53(6): 1245-1257
  2. Galletti, G. et al. (2020)
    Two subsets of stem-like CD8
    +
    memory T cell progenitors with distinct fate commitments in humans.
    Nat. Immunol. 21(12): 1552-1562
  3. Robertson, N. et al. (2020) Development of a novel mammalian display system for selection of antibodies against membrane proteins. J. Biol. Chem. 295(52): 18436-18448
  4. Formenti, S. C. et al. (2018) Radiotherapy Induces Responses of Lung Cancer to CTLA-4 Blockade. Nat Med 24(12): 1845-1851
  5. Arcangeli, S. et al. (2020) Next-Generation Manufacturing Protocols Enriching T SCM CAR T Cells Can Overcome Disease-Specific T Cell Defects in Cancer Patients. Front Immunol 11: 1217
  6. Lee, S. W. L. et al. (2018) Characterizing the Role of Monocytes in T Cell Cancer Immunotherapy Using a 3D Microfluidic Model. Front Immunol 9: 416
  7. Nicolas-Boluda, A. et al. (2021) Tumor stiffening reversion through collagen crosslinking inhibition improves T cell migration and anti-PD-1 treatment. Elife 10: e58688
  8. Alzubi, J. et al. (2020) PSMA-Directed CAR T Cells Combined with Low-Dose Docetaxel Treatment Induce Tumor Regression in a Prostate Cancer Xenograft Model. Mol. Ther. Oncolytics 18: 226-235
  9. Alzubi, J. et al. (2020) Automated generation of gene-edited CAR T cells at clinical scale. Mol Ther Methods Clin Dev. 20: 379-388
  10. Ishihara, K. et al. (1991) Stromal-cell and cytokine-dependent lymphocyte clones which span the pre-B-to B-cell transition. Dev. Immunol. 1: 149-161
  11. Dietz, L. et al. (2010) Tracking human contact allergens: from mass spectrometric identification of peptide-bound reactive small chemicals to chemical-specific naive human T-cell priming. Toxicol. Sci. 117(2): 336-347
  12. Dussiau, C. et al. (2015) Targeting IRAK1 in T-cell acute lymphoblastic leukemia. Oncotarget. 6: 18956-18965
  13. Engelmann, S. et al. (2013) T cell-independent modulation of experimental autoimmune encephalomyelitis in ADAP-deficient mice. J. Immunol. 191(10): 4950-4959
  14. Laurenti, E. et al. (2015) CDK6 levels regulate quiescence exit in human hematopoietic stem cells. Cell Stem Cell 16(3): 302-313
  15. Velardi, E. et al. (2014) Sex steroid blockade enhances thymopoiesis by modulating Notch signaling. J. Exp. Med. 211(12): 2341-2349

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