Clone:
REA714
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
B-cell activation protein

Extended validation for CD83 Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA714
HB15e++
HB15++
Cells were incubated with an excess of purified unconjugated CD83 (REA714) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD83. Mature MoDc cells were stained with CD83 antibodies and with a suitable counterstaining. As a control, CD83 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD83. Mature MoDc cells were stained with CD83 antibodies and with a suitable counterstaining. As a control, CD83 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD83. Mature MoDc cells were stained with CD83 antibodies and with a suitable counterstaining. As a control, CD83 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD83. Mature MoDc cells were stained with CD83 antibodies and with a suitable counterstaining. As a control, CD83 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD83 (REA714). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD83 (REA714). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD83 (REA714). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD83 Antibody, anti-human, REAfinity™

Overview

Clone REA714 recognizes the human CD83 antigen, a 45 kDa glycoprotein member of the immunoglobulin superfamily of receptors. In humans, CD83 is expressed on the surface of activated dendritic cells (DCs) and can therefore be used as a marker for the phenotypical characterization of DC maturation. CD83 plays a role in antigen presentation and lymphocyte activation.
Additional information: Clone REA714 displays negligible binding to Fc receptors.

Alternative names

B-cell activation protein

Detailed product information

Technical specifications

CloneREA714
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
Cross-reactivity
cynomolgus monkey (
Macaca fascicularis
)
,
rhesus monkey (
Macaca mulatta
)
,
chimpanzee (
Pan troglodytes
)
, baboon, horse
AntigenCD83
Alternative names of antigenB-cell activation protein
Molecular mass of antigen [kDa]21
Distribution of antigenlymphocytes
Entrez Gene ID9308
RRIDAB_2659321, AB_2659322, AB_2659323, AB_2659324, AB_2659325, AB_2659326, AB_2659327, AB_2905126, AB_2905125, AB_2659319, AB_2659320, AB_2659318

Resources for CD83 Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD83 Antibody, anti-human, REAfinity™

Publications

  1. Zhou, L.-J. et al. (1992) A novel cell-surface molecule expressed by human interdigitating reticulum cells, Langerhans cells, and activated lymphocytes is a new member of J. Immunol. 149: 735-742
  2. Sallusto, F. and Lanzavecchia, A. (1994) Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte/macrophage colony-stimulating factor plus interleukin 4 and downregulated by tumor necrosis factor alpha. J. Exp. Med. 179: 1109-1118
  3. Zhou, L.-J. and Tedder, T. F. (1995) Human blood dendritic cells selectively express CD83, a member of the immunoglobulin superfamily. J. Immunol. 154: 3821-3835
  4. Lechmann, M. et al. (2002) CD83 on dendritic cells: more than just a marker for maturation. Trends Immunol. 23: 273-275
  5. Cao, W. et al. (2005) CD83 is preformed inside monocytes, macrophages and dendritic cells, but it is only stably expressed on activated dendritic cells. Biochem. J. 385: 85-93

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