Clone:
REA389
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
SELP, CD62, GMP-140, GRMP, LECAM3, PADGEM, PSEL, P-selectin

Extended validation for CD62P Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA389
AK-4++
AC1.2-
Psel.KO2.3-
Cells were incubated with an excess of purified unconjugated CD62P (REA389) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD62P. Human platlets stimulated with thrombin for 4 hours were stained with CD62P antibodies and with a suitable counterstaining. As a control, CD62P antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD62P. Human platlets stimulated with thrombin for 4 hours were stained with CD62P antibodies and with a suitable counterstaining. As a control, CD62P antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD62P. Human platlets stimulated with thrombin for 4 hours were stained with CD62P antibodies and with a suitable counterstaining. As a control, CD62P antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD62P. Human platlets stimulated with thrombin for 4 hours were stained with CD62P antibodies and with a suitable counterstaining. As a control, CD62P antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for CD62P Antibody, anti-human, REAfinity™

Overview

Clone REA389 recognizes the human CD62P antigen, a 140 kDa single-pass type I membrane protein also known as P-selectin or platelet activation dependent granule-external membrane protein (PADGEM). CD62P is expressed by vascular endothelial cells and platelets. The protein is stored preformed in the Weibel-Palade bodies of endothelial cells and in the α-granules of platelets. Upon stimulation of these cells with a variety of agonists such as thrombin or histamine, CD62P is rapidly translocated to the cell surface, where it mediates leukocyte-platelet and leukocyte-vascular endothelial cell adhesion. The interaction of CD62P with mature leukocytes is in part responsible for mediating leukocyte rolling on vascular cells – an early event in leukocyte recruitment at sites of inflammation. In addition to its role as a receptor for mature neutrophils and monocytes, CD62P also functions as a cell adhesion molecule for leukocyte precursor cells in the bone marrow, including both lineage-restricted clonogenic progenitors and hierarchically more primitive progenitor cells.
Additional information: Clone REA389 displays negligible binding to Fc receptors.

Alternative names

SELP, CD62, GMP-140, GRMP, LECAM3, PADGEM, PSEL, P-selectin

Detailed product information

Technical specifications

CloneREA389
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, non-human primate
Cross-reactivity
chimpanzee (
Pan troglodytes
)
AntigenCD62P
Alternative names of antigenSELP, CD62, GMP-140, GRMP, LECAM3, PADGEM, PSEL, P-selectin
Molecular mass of antigen [kDa]86.2
Distribution of antigenendothelial cells, platelets
Entrez Gene ID6403
RRIDAB_2733831, AB_2733393, AB_2733394, AB_2784266, AB_2784265, AB_2658890, AB_2658891, AB_2733830

Resources for CD62P Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD62P Antibody, anti-human, REAfinity™

Publications

  1. Zannettino, A. C. et al. (1995) Primitive human hematopoietic progenitors adhere to P-selectin (CD62P). Blood 3466(3477): 85-12
  2. Johnston, G. I. et al. (1989) Cloning of GMP-140, a granule membrane protein of platelets and endothelium: sequence similarity to proteins involved in cell adhesion and inflammation. Cell 56(6): 1033-1044
  3. Wickenhauser, C. et al. (2000) Selectins (CD62L, CD62P) and megakaryocytic glycoproteins (CD41a, CD42b) mediate megakaryocyte-fibroblast interactions in human bone marrow. Leuk. Res. 24(12): 1013-1021

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