Name: CD27 MicroBeads, human
Availability: InStock

Product data and images for CD27 MicroBeads, human

Figures

Figure 1

Positive selection of CD19

⁺

CD27

⁺

memory B cells from human PBMCs. CD19

⁺

B cells were pre-selected using CD19 MultiSort Kit and an LS Column. In a second step, CD27 MicroBeads and an MS Column were used to isolate the memory B cells from the enriched B cell population.

Pre-selected CD19 ⁺ cells	CD19 ⁺ CD27 ⁺ cells
View details Figure 1 Positive selection of CD19 ⁺ CD27 ⁺ memory B cells from human PBMCs. CD19 ⁺ B cells were pre-selected using CD19 MultiSort Kit and an LS Column. In a second step, CD27 MicroBeads and an MS Column were used to isolate the memory B cells from the enriched B cell population.	View details Figure 1 Positive selection of CD19 ⁺ CD27 ⁺ memory B cells from human PBMCs. CD19 ⁺ B cells were pre-selected using CD19 MultiSort Kit and an LS Column. In a second step, CD27 MicroBeads and an MS Column were used to isolate the memory B cells from the enriched B cell population.

Specifications for CD27 MicroBeads, human

Overview

CD27 MicroBeads were developed for the positive selection or depletion of CD27

⁺

cells from human PBMCs or lymphoid tissues. CD27 is differentially expressed on B cells, T cells, and NK cells.

Detailed product information

Background information

CD27 is a member of the tumor necrosis factor receptor family, which among peripheral blood B cells is present on memory B cells

and plasma cells but not on naive B cells. In human tonsils, expression of the CD27 antigen is found on memory B cells, germinal center B cells, subepithelial marginal zone B cells, and plasma cells. CD27 is further expressed on specific T cell subsets and NK cell subsets.

Applications

T cells can be pre-selected using the CD8

⁺

T Cell Isolation Kit or the CD4

⁺

T Cell Isolation Kit and subsequently separated according to CD27 expression.

For the isolation of human memory B cells, we recommend the Memory B Cell Isolation Kit.

Columns

For positive selection: MS, LS, XS, or autoMACS

^®

Columns. For depletion: LD, D, or autoMACS Columns.

References for CD27 MicroBeads, human

Publications

van Lier, R. A. et al. (1987) Tissue distribution and biochemical and functional properties of Tp55 (CD27), a novel T cell differentiation antigen. J. Immunol. 139: 1589-1596
Bigler, R. D. et al. (1988) S152 (CD27). A modulating disulfide-linked T cell activation antigen. J. Immunol. 139: 21-28
Klein, U. et al. (1998)
Human immunoglobulin (Ig)M
⁺
IgD
⁺
peripheral blood B cells expressing the CD27 cell surface antigen carry somatically mutated variable region genes: CD27 as a general marker for somatically mutated (memory) B cells.
J. Exp. Med. 188: 1679-1689
Tangye, S. G. et al. (1998) Identification of functional human splenic memory B cells by expression of CD148 and CD27. J. Exp. Med. 188: 1691-1703
Hintzen, R. Q. et al. (1993) Regulation of CD27 expression on subsets of mature T-lymphocytes. J. Immunol. 151: 2426-2435
Elson, L. H. et al. (1995)
Flow cytometric analysis for cytokine production identifies T helper 1, T helper 2, and T helper 0 cells within the human CD4
⁺
CD27
^–
lymphocyte subpopulation.
J. Immunol. 154: 4294-4301
Hintzen, R. Q. et al. (1994) Characterization of the human CD27 ligand, a novel member of the TNF gene family. J. Immunol. 152: 1762-1773
Hamann, D. et al. (1997)
Phenotypic and functional separation of memory and effector human CD8
⁺
T cells.
J. Exp. Med. 186: 1407-1418
De Milito, A. et al. (2004) Mechanisms of hypergammaglobulinemia and impaired antigen-specific humoral immunity in HIV-1 infection. Blood 103: 2180-2186

Data and images

Data and images for CD27 MicroBeads, human

Figures

Figure 1

Positive selection of CD19

⁺

CD27

⁺

memory B cells from human PBMCs. CD19

⁺

B cells were pre-selected using CD19 MultiSort Kit and an LS Column. In a second step, CD27 MicroBeads and an MS Column were used to isolate the memory B cells from the enriched B cell population.

Pre-selected CD19 ⁺ cells	CD19 ⁺ CD27 ⁺ cells
View details Figure 1 Positive selection of CD19 ⁺ CD27 ⁺ memory B cells from human PBMCs. CD19 ⁺ B cells were pre-selected using CD19 MultiSort Kit and an LS Column. In a second step, CD27 MicroBeads and an MS Column were used to isolate the memory B cells from the enriched B cell population.	View details Figure 1 Positive selection of CD19 ⁺ CD27 ⁺ memory B cells from human PBMCs. CD19 ⁺ B cells were pre-selected using CD19 MultiSort Kit and an LS Column. In a second step, CD27 MicroBeads and an MS Column were used to isolate the memory B cells from the enriched B cell population.

Specifications

Specifications for CD27 MicroBeads, human

Overview

CD27 MicroBeads were developed for the positive selection or depletion of CD27

⁺

cells from human PBMCs or lymphoid tissues. CD27 is differentially expressed on B cells, T cells, and NK cells.

Detailed product information

Background information

CD27 is a member of the tumor necrosis factor receptor family, which among peripheral blood B cells is present on memory B cells

Applications

T cells can be pre-selected using the CD8

⁺

T Cell Isolation Kit or the CD4

⁺

T Cell Isolation Kit and subsequently separated according to CD27 expression.

For the isolation of human memory B cells, we recommend the Memory B Cell Isolation Kit.

Columns

For positive selection: MS, LS, XS, or autoMACS

^®

Columns. For depletion: LD, D, or autoMACS Columns.

References

References for CD27 MicroBeads, human

Publications

van Lier, R. A. et al. (1987) Tissue distribution and biochemical and functional properties of Tp55 (CD27), a novel T cell differentiation antigen. J. Immunol. 139: 1589-1596
Bigler, R. D. et al. (1988) S152 (CD27). A modulating disulfide-linked T cell activation antigen. J. Immunol. 139: 21-28
Klein, U. et al. (1998)
Human immunoglobulin (Ig)M
⁺
IgD
⁺
peripheral blood B cells expressing the CD27 cell surface antigen carry somatically mutated variable region genes: CD27 as a general marker for somatically mutated (memory) B cells.
J. Exp. Med. 188: 1679-1689
Tangye, S. G. et al. (1998) Identification of functional human splenic memory B cells by expression of CD148 and CD27. J. Exp. Med. 188: 1691-1703
Hintzen, R. Q. et al. (1993) Regulation of CD27 expression on subsets of mature T-lymphocytes. J. Immunol. 151: 2426-2435
Elson, L. H. et al. (1995)
Flow cytometric analysis for cytokine production identifies T helper 1, T helper 2, and T helper 0 cells within the human CD4
⁺
CD27
^–
lymphocyte subpopulation.
J. Immunol. 154: 4294-4301
Hintzen, R. Q. et al. (1994) Characterization of the human CD27 ligand, a novel member of the TNF gene family. J. Immunol. 152: 1762-1773
Hamann, D. et al. (1997)
Phenotypic and functional separation of memory and effector human CD8
⁺
T cells.
J. Exp. Med. 186: 1407-1418
De Milito, A. et al. (2004) Mechanisms of hypergammaglobulinemia and impaired antigen-specific humoral immunity in HIV-1 infection. Blood 103: 2180-2186

CD27 MicroBeads, human

CD27 MicroBeads, human

Product overview

Product data and images for CD27 MicroBeads, human

Figures

Figure 1

Figure 1

Figure 1

Specifications for CD27 MicroBeads, human

Overview

Detailed product information

Background information

Applications

Columns

References for CD27 MicroBeads, human

Publications

Data and images

Data and images for CD27 MicroBeads, human

Figures

Figure 1

Figure 1

Figure 1

Specifications

Specifications for CD27 MicroBeads, human

Overview

Detailed product information

Background information

Applications

Columns

References

References for CD27 MicroBeads, human

Publications

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CD27 MicroBeads, human

CD27 MicroBeads, human

Product overview

Product data and images for CD27 MicroBeads, human

Figures

Figure 1

Figure 1

Figure 1

Specifications for CD27 MicroBeads, human

Overview

Detailed product information

Background information

Applications

Columns

References for CD27 MicroBeads, human

Publications

Data and images for CD27 MicroBeads, human

Figures

Figure 1

Figure 1

Figure 1

Specifications for CD27 MicroBeads, human

Overview

Detailed product information

Background information

Applications

Columns

References for CD27 MicroBeads, human

Publications

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