Clone:
REA1222
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC, MC
Alternative names:
FCER2, FcεRII, CD23A, CLEC4J, FCE2, IGEBF

Extended validation for CD23 Antibody, anti-human, REAfinity™

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD23. Human peripheral blood mononuclear cells (PBMCs) were stained with CD23 antibodies and with a suitable counterstaining. As a control, CD23 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD23. Human peripheral blood mononuclear cells (PBMCs) were stained with CD23 antibodies and with a suitable counterstaining. As a control, CD23 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD23. Human peripheral blood mononuclear cells (PBMCs) were stained with CD23 antibodies and with a suitable counterstaining. As a control, CD23 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD23. Human peripheral blood mononuclear cells (PBMCs) were stained with CD23 antibodies and with a suitable counterstaining. As a control, CD23 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD23. Human peripheral blood mononuclear cells (PBMCs) were stained with CD23 antibodies and with a suitable counterstaining. As a control, CD23 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD23. Human peripheral blood mononuclear cells (PBMCs) were stained with CD23 antibodies and with a suitable counterstaining. As a control, CD23 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD23. Human peripheral blood mononuclear cells (PBMCs) were stained with CD23 antibodies and with a suitable counterstaining. As a control, CD23 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD23. Human peripheral blood mononuclear cells (PBMCs) were stained with CD23 antibodies and with a suitable counterstaining. As a control, CD23 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD23. Human peripheral blood mononuclear cells (PBMCs) were stained with CD23 antibodies and with a suitable counterstaining. As a control, CD23 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD23. Human peripheral blood mononuclear cells (PBMCs) were stained with CD23 antibodies and with a suitable counterstaining. As a control, CD23 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD23. Human peripheral blood mononuclear cells (PBMCs) were stained with CD23 antibodies and with a suitable counterstaining. As a control, CD23 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD23 (REA1222). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD23 (REA1222). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD23 (REA1222). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD23 (REA1222). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD23 (REA1222). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD23 Antibody, anti-human, REAfinity™

Overview

Clone REA1222 recognizes the human CD23 antigen, which is a 45 kDa type II transmembrane glycoprotein that belongs to the immunoglobulin family. It is expressed on most mature B cells, monocytes, follicular dendritic cells, and at low levels on T cells, Langerhans cells, eosinophils, and platelets. CD23 is thought to play a role in the regulation of B cell activation and IgE response. It is up-regulated upon B cell activation. The proteolytic cleavage of membrane CD23 can result in the release of soluble forms of the antigen (sCD23) with a reported biological activity.
Additional information: Clone REA1222 displays negligible binding to Fc receptors.

Alternative names

FCER2, FcεRII, CD23A, CLEC4J, FCE2, IGEBF

Detailed product information

Technical specifications

CloneREA1222
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD23
Alternative names of antigenFCER2, FcεRII, CD23A, CLEC4J, FCE2, IGEBF
Molecular mass of antigen [kDa]36
Distribution of antigenB cells, dendritic cells, endothelial cells, Langerhans cells, macrophages, monocytes, NK cells, platelets, T cells, eosinophils, neutrophils, thymocytes
Entrez Gene ID2208
RRIDAB_2811604, AB_2811612, AB_2811605, AB_2811613, AB_2811606, AB_2811614, AB_2811607, AB_2811615, AB_2811608, AB_2811616, AB_2811609, AB_2811617, AB_2811610, AB_2889518, AB_2904862, AB_2904861, AB_2811611

Resources for CD23 Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

Related products for
CD23 Antibody, anti-human, REAfinity™

1 products available

Seems like you are coming from USA!
Do you want to visit our website in your country?