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Separation of bone marrow cells using CD117 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with lineage markers (biotinylated antibodies against CD5, CD11b, CD45R, Gr-1, 7-4, Ter-119 and Anti-Biotin-APC) and CD117-PE. |
A: | B: |
Before enrichment | Isolated CD117 + cells after enrichment |
CD117 MicroBeads, mouseFigure 1Separation of bone marrow cells using CD117 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with lineage markers (biotinylated antibodies against CD5, CD11b, CD45R, Gr-1, 7-4, Ter-119 and Anti-Biotin-APC) and CD117-PE. | CD117 MicroBeads, mouseFigure 1Separation of bone marrow cells using CD117 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with lineage markers (biotinylated antibodies against CD5, CD11b, CD45R, Gr-1, 7-4, Ter-119 and Anti-Biotin-APC) and CD117-PE. |
It has been shown that CD117+ is a marker of prostate basal stem cells. We used this anti-mouse CD117 magnetic microbeads to sort cells from the prostate, cells that constitute the putative population of basal cells that can reconstitute prostatic ducts. This research aimed to characterize the cell type(s) in the prostate that express a key marker of murine and human ESCs, and explore the hypothesis that aberrant expression of this marker may contribute to prostate inflammation/hyperplasia sometimes associated with prostatitis. We recommend this product.
Separation of bone marrow cells using CD117 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with lineage markers (biotinylated antibodies against CD5, CD11b, CD45R, Gr-1, 7-4, Ter-119 and Anti-Biotin-APC) and CD117-PE. |
A: | B: |
Before enrichment | Isolated CD117 + cells after enrichment |
CD117 MicroBeads, mouseFigure 1Separation of bone marrow cells using CD117 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with lineage markers (biotinylated antibodies against CD5, CD11b, CD45R, Gr-1, 7-4, Ter-119 and Anti-Biotin-APC) and CD117-PE. | CD117 MicroBeads, mouseFigure 1Separation of bone marrow cells using CD117 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with lineage markers (biotinylated antibodies against CD5, CD11b, CD45R, Gr-1, 7-4, Ter-119 and Anti-Biotin-APC) and CD117-PE. |
It has been shown that CD117+ is a marker of prostate basal stem cells. We used this anti-mouse CD117 magnetic microbeads to sort cells from the prostate, cells that constitute the putative population of basal cells that can reconstitute prostatic ducts. This research aimed to characterize the cell type(s) in the prostate that express a key marker of murine and human ESCs, and explore the hypothesis that aberrant expression of this marker may contribute to prostate inflammation/hyperplasia sometimes associated with prostatitis. We recommend this product.
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