Find the products and resources you are looking for!
Miltenyi Biotec distribution:
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
|Column||Max. number of labeled cells||Max. number of total cells||Separator|
|Positive selection or depletion|
Coat a 24-well culture dish with 0.01% Poly-L-lysine overnight at 37 °C. Wash three times with ddH₂O the following day.
Prepare the following cell culture medium: DMEM containing 10% FBS, 1% penicillin/streptomycin, and 2mM L-glutamine.
Use the Neural Tissue Dissociation Kit (T) or the Neural Tissue Dissociation Kit (P) to dissociate brain tissue and prepare a single-cell suspension. Follow the protocol of the kit data sheet.
Download data sheet
Isolate microglia from the single-cell suspension using either the CD11b (Microglia) MicroBeads, human and mouse or the CD11b/c (Microglia) MicroBeads, rat. Follow the protocol of the corresponding kit data sheet.
Download data sheet
CD11b+ microglia isolated from neonatal mouse brain tissue. CD11b+ cells were isolated from mouse neural cell suspension using the CD11b (Microglia) MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with CD11b-APC and CD45-FITC. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Before MicroBead labeling
Isolated CD11b/c+ cells
CD11b/c+ microglia isolated from neonatal rat brain tissue. CD11b/c+ cells were isolated from P3 rat brain tissue using the Neural Tissue Dissociation Kit (P), the gentleMACS™ Dissociator, CD11b/c (Microglia) MicroBeads, a MiniMACS™ Separator, and two MS Columns. Cells were fluorecently stained with CD11b/c-FITC in combination with CD45-APC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use.
autoMACS, CliniMACS, the CliniMACS logo, CliniMACS Prodigy, CryoMACS, CytoMix, CytoStim, DendriMACS, ExiTron, ExpAct, FeraSpin, FeraTrack, GadoSpin, gentleMACS, LIFE 18, LIFE 21, MACS, the MACS logo, MACSductin, MACSelect, MACSfectin, MACSflex, MACSiBead, MACSiMAG, MACSmix, MACSprep, MACSQuant, MACSQuantify, MACSxpress, MidiMACS, MiniMACS, miRXplore, MultiMACS, NeuroBrew, NiraWave, OctoMACS, PepTivator, pMACS, PolySon, PrepProtect, QuadroMACS, REAfinity, REAlease, Rheo, StemMACS, StraightFrom, SuperAmp, SuperMACS, TexMACS, TheraSorb, thermoMACS, TransAct, Tyto, the Tyto logo, VarioMACS, Vio, Viobility, VioBlue, VioBright, VioGreen, Viscover, and µMACS are registered trademarks or trademarks of Miltenyi Biotec B.V. & Co. KG and/or its affiliates in various countries worldwide. Ficoll-Paque is a trademark of GE Healthcare companies. Vectofusin-1 is a registered trademark of Genethon. All other trademarks mentioned in this document are the property of their respective owners and are used for identification purposes only. Copyright © 2019 Miltenyi Biotec B.V. & Co. KG and/or its affiliates. All rights reserved.
Copyright © 2019 Miltenyi Biotec and/or its affiliates. All rights reserved.