Clone:
REA633
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
TCRgd

Extended validation for TCRγ/δ Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA633
GL3++
UC7-13D5++
Cells were incubated with an excess of purified unconjugated TCRγ/δ (REA633) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for Anti-TCRγ/δ. Splenocytes from CD1 mice were stained with Anti-TCRγ/δ antibodies and with a suitable counterstaining. As a control, Anti-TCRγ/δ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-TCRγ/δ. Splenocytes from CD1 mice were stained with Anti-TCRγ/δ antibodies and with a suitable counterstaining. As a control, Anti-TCRγ/δ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-TCRγ/δ. Splenocytes from CD1 mice were stained with Anti-TCRγ/δ antibodies and with a suitable counterstaining. As a control, Anti-TCRγ/δ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-TCRγ/δ. Splenocytes from CD1 mice were stained with Anti-TCRγ/δ antibodies and with a suitable counterstaining. As a control, Anti-TCRγ/δ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-TCRγ/δ. Splenocytes from CD1 mice were stained with Anti-TCRγ/δ antibodies and with a suitable counterstaining. As a control, Anti-TCRγ/δ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for Anti-TCRγ/δ. Splenocytes from CD1 mice were stained with Anti-TCRγ/δ antibodies and with a suitable counterstaining. As a control, Anti-TCRγ/δ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using TCRγ/δ (REA633). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using TCRγ/δ (REA633). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using TCRγ/δ (REA633). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for TCRγ/δ Antibody, anti-mouse, REAfinity™

Overview

Clone REA633 recognizes the mouse TCRγ/δ antigen. The T cell receptor (TCR) is a heterodimeric glycoprotein associated with the CD3 antigen. It consists of an α and a β chain (TCRα/β) or a γ and a δ chain (TCRγ/δ). The γ and δ TCR chains are composed of constant and variable regions, each encoded by distinct gene segments. The γ chain forms either disulfide-linked or non-disulfide-linked heterodimers with the δ-subunit. The γ/δ T cell receptor is present on a subset of T lymphocytes in peripheral blood. TCRγ/δ is involved in the antigen recognition of tumor-associated antigens or bacterial antigens presented by MHC class I molecules.
Additional information: Clone REA633 displays negligible binding to Fc receptors.

Alternative names

TCRgd

Detailed product information

Technical specifications

CloneREA633
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenTCRγ/δ
Alternative names of antigenTCRgd
Distribution of antigenT cells
Entrez Gene ID110066, 110067
RRIDAB_2751405, AB_2801845, AB_2802022, AB_2654049, AB_2654050, AB_2725820, AB_2725818, AB_2654057, AB_2654058, AB_2751422

References for TCRγ/δ Antibody, anti-mouse, REAfinity™

Publications

  1. Schmolka, N. et al. (2013) Epigenetic and transcriptional signatures of stable versus plastic differentiation of proinflammatory γδ T cell subsets. Nat. Immunol. 14(10): 1093-1100
  2. Bonneville, M. et al. (2010) Gammadelta T cell effector functions: a blend of innate programming and acquired plasticity. Nat. Rev. Immunol. 10(7): 467-478
  3. Wiest, D. L. (2016) Development of γδ T cells, the special-force soldiers of the immune system. Methods Mol. Biol. 1323: 23-32

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