Clone:
11F2
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1κ
Applications:
FC
Alternative names:
TRD, TCRD, TCRDV1, TRD@, TCRG, TRG@, TRG

Extended validation for TCRγ/δ Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with 11F2
B1+
REA591++
IMMU510++
Cells were incubated with an excess of purified unconjugated TCRγ/δ (11F2) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for Anti-TCRγ/δ. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-TCR/γδ antibodies and with a suitable counterstaining. As a control, Anti-TCRγ/δ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-TCRγ/δ. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-TCR/γδ antibodies and with a suitable counterstaining. As a control, Anti-TCRγ/δ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-TCRγ/δ. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-TCR/γδ antibodies and with a suitable counterstaining. As a control, Anti-TCRγ/δ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-TCRγ/δ. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-TCR/γδ antibodies and with a suitable counterstaining. As a control, Anti-TCRγ/δ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-TCRγ/δ. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-TCR/γδ antibodies and with a suitable counterstaining. As a control, Anti-TCRγ/δ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-TCRγ/δ. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-TCR/γδ antibodies and with a suitable counterstaining. As a control, Anti-TCRγ/δ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for Anti-TCRγ/δ. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-TCR/γδ antibodies and with a suitable counterstaining. As a control, Anti-TCRγ/δ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using TCRγ/δ (11F2). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using TCRγ/δ (11F2). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using TCRγ/δ (11F2). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for TCRγ/δ Antibody, anti-human

Overview

The T cell receptor is a heterodimeric glycoprotein associated with the CD3 antigen. The TCR consists of an α and a β chain (TCRα/β) or a γ and a δ chain (TCRγ/δ). Clone 11F2 reacts with a framework epitope of the γ/δ T cell–receptor. The γ and δ TCR chains are composed of constant and variable regions, each encoded by distinct gene segments. The γ chain forms either disulfide-linked or non-disulfide-linked heterodimers with the δ-subunit.The γ/δ Tcell–receptor is present on a subset of T lymphocytes in peripheral blood, intestinal epithelium, lymph node, thymus, and spleen. TCR γ/δ is involved in the recognition of certain bacterial, self-CD1 molecule, and tumor antigens bound to MHC class I. γ/δ T cells are mainly CD4 negative and CD8 negative. T cells expressing the γ/δ TCR have been shown to play a role in oral tolerance, innate immune response for some tumor cells, and autoimmune disease. Antigen presentation by γ/δ T cells has been reported.

Alternative names

TRD, TCRD, TCRDV1, TRD@, TCRG, TRG@, TRG

Detailed product information

Technical specifications

Clone11F2
Clonalitymonoclonal
Isotypemouse IgG1κ
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman
AntigenTCRγ/δ
Alternative names of antigenTRD, TCRD, TCRDV1, TRD@, TCRG, TRG@, TRG
Distribution of antigenT cells
Entrez Gene ID6965
RRIDAB_2733699, AB_2733904, AB_2733905, AB_2733462, AB_2733463, AB_2733976, AB_2733977, AB_2733287, AB_2733288, AB_2751186, AB_2751120, AB_2733075, AB_2733076, AB_2733574, AB_2733575, AB_2733698

References for TCRγ/δ Antibody, anti-human

Publications

  1. Spencer, C. T. et al. (2008) Only a subset of phosphoantigen-responsive gamma9delta2 T cells mediate protective tuberculosis immunity. J. Immunol. 181(7): 4471-4484
  2. Voogt, P. J. et al. (1989)
    Normal hematopoietic progenitor cells and malignant lymphohematopoietic cells show different susceptibility to direct cell-mediated MHC-non-restricted lysis by T cell receptor
    /CD3
    , T cell receptor gamma delta
    +
    /CD3
    +
    and T cell receptor-alpha beta
    +
    /CD3
    +
    lymphocytes.
    J. Immunol. 142(5): 1774-1780
  3. Borst, J. et al. (1988) Distinct molecular forms of human T cell receptor gamma/delta detected on viable T cells by a monoclonal antibody. J. Exp. Med. 167(5): 1625-1644
  4. Lanier, L. L. et al. (1987)
    The T cell antigen receptor complex expressed on normal peripheral blood CD4
    , CD8
    T lymphocytes. A CD3-associated disulfide-linked gamma chain heterodimer.
    J. Exp. Med. 165(4): 1076-1094
  5. Conrad, M. L. et al. (2007) TCR and CD3 antibody cross-reactivity in 44 species. Cytometry A 71(11): 925-933
  6. Lanier, L. L. et al. (1988) Structural and serological heterogeneity of γ/δ T cell antigen receptor expression in thymus and peripheral blood. Eur. J. Immunol. 18(12): 1985-1992
  7. Testi, R. and Lanier, L. L. (1989) Functional expression of CD28 on T cell antigen receptor gamma/delta-bearing T lymphocytes. Eur. J. Immunol. 19(1): 185-188
  8. Lanier, L. L. et al. (1987) The gamma T-cell antigen receptor. J. Clin. Immunol. 7(6): 429-440
  9. Moens, E. et al. (2011) IL-23R and TCR signaling drives the generation of neonatal Vγ9 Vδ2 T cells expressing high levels of cytotoxic mediators and producing IFN-γ and IL-17. J. Leukoc. Biol. 89(5): 743-752

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