StemMACS™ Purmorphamine is a small molecule activator of hedgehog signaling.

Data and images for StemMACS™ Purmorphamine

Figures

Figure

View details
Chemical structure of StemMACS™ Purmorphamine
Chemical structure of StemMACS™ Purmorphamine

Specifications for StemMACS™ Purmorphamine

Overview

StemMACS™ Purmorphamine is a small molecule activator of hedgehog signaling.

Detailed product information

Background information

StemMACS™ Purmorphamine is a small molecule that activates the Hedgehog signaling pathway by binding to the smoothened receptor (IC50 ~ 1.5 μM). Binding is competitive with respect to the smoothened antagonist cyclopamine. Purmorphamine has been used instead of sonic hedgehog for motor neuron differentiation and can promote osteogenesis in mesenchymal progenitor cells.

Applications

Published applications for Purmorphamine include:
  • Activation of Hedgehog signaling1
  • Motor neuron differentiation from pluripotent stem cells2,3
  • Osteoblast differentiation from mesenchymal progenitors4,5,6
  • Adipocyte differentiation from mesenchymal progenitors7

Resources for StemMACS™ Purmorphamine

Documents and Protocols

References for StemMACS™ Purmorphamine

Publications

  1. Sinha, S. et al. (2006) Purmorphamine activates the Hedgehog pathway by targeting Smoothened. Nat. Chem. Biol. 2(1): 29-30
  2. Li, X. J. et al. (2008) Directed differentiation of ventral spinal progenitors and motor neurons from human embryonic stem cells by small molecules. Stem Cells 26(4): 886-893
  3. Hu, B.Y. et al. (2009) Differentiation of spinal motor neurons from pluripotent human stem cells. Nat. Protoc. 4(9): 1295-1304
  4. Beloti et al. (2005) Int J Cell Biol 29(7): 537-541
  5. Wu, X. et al. (2002) A small molecule with osteogenesis-inducing activity in multipotent mesenchymal progenitor cells. J. Am. Chem. Soc. 124: 14520-14521
  6. Wu et al. (2004) Purmorphamine induces osteogenesis by activation of the hedgehog signaling pathway. Chem. Biol. 11: 1229-1238
  7. Fontaine, C. et al. (2008) Hedgehog signaling alters adipocyte maturation of human mesenchymal stem cells. Stem Cells 26(4): 1037-1046