StemMACS™ DiffBase XF (xeno-free) is an optimized and standardized base medium for human pluripotent stem cells (hPSCs) differentiation.

Data and images for StemMACS™ DiffBase XF, human

Figures

Figure 1: Optimized for hPSCs cultivated in iPS-Brew XF

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Thanks to biochemical and physicochemical characteristics similar to iPS-Brew XF, StemMACS™ DiffBase XF allows a smooth and gentle transition into the differentiation protocol without the need to adapt the cells to the new media. This results in a shorter cultivation time and less stress for the culture.
Thanks to biochemical and physicochemical characteristics similar to iPS-Brew XF, StemMACS™ DiffBase XF allows a smooth and gentle transition into the differentiation protocol without the need to adapt the cells to the new media. This results in a shorter cultivation time and less stress for the culture.

Figure 2: Supports directed differentiation in different culture conditions

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StemMACS™ DiffBase XF can be used as base media for any type of directed differentiation after supplementation with the appropriate patterning cytokines and small molecules. The robust formulation of StemMACS DiffBase XF supports optimal cell growth both in directed differentiation protocols based on monolayer adherent cultures and in protocols based on 3D culture.
Cultivation in StemMACS DiffBase XF supplemented with germ-layer specific patterning factors results in expression of early differentiation markers already after 6 days, as demonstrated by flow cytometry.
StemMACS™ DiffBase XF can be used as base media for any type of directed differentiation after supplementation with the appropriate patterning cytokines and small molecules. The robust formulation of StemMACS DiffBase XF supports optimal cell growth both in directed differentiation protocols based on monolayer adherent cultures and in protocols based on 3D culture.
Cultivation in StemMACS DiffBase XF supplemented with germ-layer specific patterning factors results in expression of early differentiation markers already after 6 days, as demonstrated by flow cytometry.

Figure 3: Sustains embryoid bodies formations and culture

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Figure 4: Suitable for hPSCs-pluripotency assessment by embryoid bodies assay

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StemMACS™ DiffBase XF supports cell survival and multiplication also without cytokines addition and can be therefore additionally used as media to assess PSC pluripotency by embryoid body assay. In fact, StemMACS DiffBase XF supports the formation and long-term survival of embryoid bodies (EBs, fig. 3).
EBs grown in suspension for two weeks in StemMACS DiffBase XF and for 1 week in adherent culture, show differentiation in cellular types deriving from the 3 embryonic germ layers (fig. 4).
StemMACS™ DiffBase XF supports cell survival and multiplication also without cytokines addition and can be therefore additionally used as media to assess PSC pluripotency by embryoid body assay. In fact, StemMACS DiffBase XF supports the formation and long-term survival of embryoid bodies (EBs, fig. 3).
EBs grown in suspension for two weeks in StemMACS DiffBase XF and for 1 week in adherent culture, show differentiation in cellular types deriving from the 3 embryonic germ layers (fig. 4).

Specifications for StemMACS™ DiffBase XF, human

Overview

StemMACS™ DiffBase XF (xeno-free) is an optimized and standardized base medium for human pluripotent stem cells (hPSCs) differentiation.

Detailed product information

Background information

StemMACS™ DiffBase XF (xeno-free) is an optimized and standardized base medium for the differentiation of human pluripotent stem cells (hPSCs). StemMACS DiffBase XF has been developed to be used directly on hPSCs cultivated in StemMACS iPS-Brew XF and, when supplemented with the appropriate patterning cytokines and small molecules, can be used as a base medium for virtually every type of directed differentiation protocol. Moreover, without the need for further cytokines supplementation, StemMACS DiffBase XF can be conveniently used as media to assess hPSCs pluripotency by embryoid body assay.

Applications

  • Directed differentiation of hPSCs as monolayer adherent cultures or via embryoid bodies formation (3D differentiation).
  • Pluripotency assessment by embryoid bodies assay (spontaneous differentiation)

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