StemMACS CardioDiff Kit XF is a complete ready-to-use, and xeno-free cell culture system for efficient and fast differentiation of human pluripotent stem cells (PSCs) into cardiomyocytes.

Data and images for StemMACS™ CardioDiff Kit XF, human

Figures

Figure 1

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Fast and easy protocol:
(A) StemMACS™ CardioDiff Kit XF is composed of three media that progressively restrict the cellular fate and promote the differentiation into cardiomyocytes in just 8 days of culture. PSCs-derived cardiomyocytes can be further expanded in StemMACS Cardiac Cultivation Medium and cultivated for more than 30 days.
(B) Substantial changings on cellular morphology are observable during the differentiation and, depending on the cellular line, first contracting cardiomyocytes can be observed already after 6 days of culture.

Figure 1

Fast and easy protocol:
(A) StemMACS™ CardioDiff Kit XF is composed of three media that progressively restrict the cellular fate and promote the differentiation into cardiomyocytes in just 8 days of culture. PSCs-derived cardiomyocytes can be further expanded in StemMACS Cardiac Cultivation Medium and cultivated for more than 30 days.
(B) Substantial changings on cellular morphology are observable during the differentiation and, depending on the cellular line, first contracting cardiomyocytes can be observed already after 6 days of culture.

Figure 2

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High efficiency:
StemMACS™ CardioDiff Kit XF yields to differentiation efficiencies up to 85% as measured by flow cytometry analysis of cardiac troponin T (cTnT) in different PSC lines, with good experiment-to-experiment consistency.

Figure 2

High efficiency:
StemMACS™ CardioDiff Kit XF yields to differentiation efficiencies up to 85% as measured by flow cytometry analysis of cardiac troponin T (cTnT) in different PSC lines, with good experiment-to-experiment consistency.

Figure 3

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Characterization:
(A) Characterization by flow cytometry and immunofluorescence staining of cardiomyocytes generated with StemMACS™ CardioDiff Kit XF shows the expression of typical cardiomyocytes markers (cardiac troponin T, alpha actinin, MHC, MLC2a). (B) Immunofluorescence staining for Kv1.5 and phospholamban (PLN) indicates the presence of cardiomyocytes with electrical properties and Ca
2+
handling machinery.

Figure 3

Characterization:
(A) Characterization by flow cytometry and immunofluorescence staining of cardiomyocytes generated with StemMACS™ CardioDiff Kit XF shows the expression of typical cardiomyocytes markers (cardiac troponin T, alpha actinin, MHC, MLC2a). (B) Immunofluorescence staining for Kv1.5 and phospholamban (PLN) indicates the presence of cardiomyocytes with electrical properties and Ca
2+
handling machinery.

Figure 4

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Scalable:
The protocol can be scaled-up in different cultureware formats without differences in the efficiency rates.

Figure 4

Scalable:
The protocol can be scaled-up in different cultureware formats without differences in the efficiency rates.

Specifications for StemMACS™ CardioDiff Kit XF, human

Overview

StemMACS CardioDiff Kit XF is a complete ready-to-use, and xeno-free cell culture system for efficient and fast differentiation of human pluripotent stem cells (PSCs) into cardiomyocytes.

Detailed product information

Background information

Directed differentiation of specific lineages from human pluripotent stem cells (hPSCs) is a major tool for developmental or disease models, drug screening platforms, and cellular therapies. For all these applications, it is pivotal to have a consistent, time-saving, and reliable differentiation method.
StemMACS CardioDiff Kit XF is a complete, ready-to-use, and xeno-free cell culture system for efficient and fast differentiation of hPSCs into cardiomyocytes. The kit is composed of three phenol red–free media that progressively restrict the cellular fate and promote the differentiation into cardiomyocytes in just 8 days of culture. Depending on the cellular line, first contracting cardiomyocytes can be observed already after 6 days of culture. Moreover, the protocol can be up-scaled without differences in the efficiency rates. PSCs-derived cardiomyocytes can be further expanded in StemMACS Cardiac Cultivation Medium and cultivated for more than 30 days.

Applications

  • Directed differentiation of cardiomyocytes from hPSCs lines

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