StemMACS Cardiac Cultivation Medium is a complete ready-to-use xeno-free cell culture medium for the cultivation of human cardiomyocytes.

Data and images for StemMACS™ Cardiac Cultivation Medium XF, human

Figures

Figure 1

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Fast and easy protocol:
(A) StemMACS™ CardioDiff Kit XF is composed of three media that progressively restrict the cellular fate and promote the differentiation into cardiomyocytes in just 8 days of culture. PSCs-derived cardiomyocytes can be further expanded in StemMACS Cardiac Cultivation Medium and cultivated for more than 30 days.
(B) Substantial changings on cellular morphology are observable during the differentiation and, depending on the cellular line, first contracting cardiomyocytes can be observed already after 6 days of culture.

Figure 1

Fast and easy protocol:
(A) StemMACS™ CardioDiff Kit XF is composed of three media that progressively restrict the cellular fate and promote the differentiation into cardiomyocytes in just 8 days of culture. PSCs-derived cardiomyocytes can be further expanded in StemMACS Cardiac Cultivation Medium and cultivated for more than 30 days.
(B) Substantial changings on cellular morphology are observable during the differentiation and, depending on the cellular line, first contracting cardiomyocytes can be observed already after 6 days of culture.

Figure 2

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High efficiency:
StemMACS™ CardioDiff Kit XF yields to differentiation efficiencies up to 85% as measured by flow cytometry analysis of cardiac troponin T (cTnT) in different PSC lines, with good experiment-to-experiment consistency.

Figure 2

High efficiency:
StemMACS™ CardioDiff Kit XF yields to differentiation efficiencies up to 85% as measured by flow cytometry analysis of cardiac troponin T (cTnT) in different PSC lines, with good experiment-to-experiment consistency.

Figure 3

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Characterization:
(A) Characterization by flow cytometry and immunofluorescence staining of cardiomyocytes generated with StemMACS™ CardioDiff Kit XF shows the expression of typical cardiomyocytes markers (cardiac troponin T, alpha actinin, MHC, MLC2a). (B) Immunofluorescence staining for Kv1.5 and phospholamban (PLN) indicates the presence of cardiomyocytes with electrical properties and Ca
2+
handling machinery.

Figure 3

Characterization:
(A) Characterization by flow cytometry and immunofluorescence staining of cardiomyocytes generated with StemMACS™ CardioDiff Kit XF shows the expression of typical cardiomyocytes markers (cardiac troponin T, alpha actinin, MHC, MLC2a). (B) Immunofluorescence staining for Kv1.5 and phospholamban (PLN) indicates the presence of cardiomyocytes with electrical properties and Ca
2+
handling machinery.

Figure 4

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Scalable:
The protocol can be scaled-up in different cultureware formats without differences in the efficiency rates.

Figure 4

Scalable:
The protocol can be scaled-up in different cultureware formats without differences in the efficiency rates.

Specifications for StemMACS™ Cardiac Cultivation Medium XF, human

Overview

StemMACS Cardiac Cultivation Medium is a complete ready-to-use xeno-free cell culture medium for the cultivation of human cardiomyocytes.

Detailed product information

Background information

Directed differentiation of specific lineages from human pluripotent stem cells (hPSCs) is a major tool for developmental or disease models, drug screening platforms, or cellular therapies from hPSCs. In this regard, cardiomyocytes generated from pluripotent stem cells are widely used as a model system in basic and translational research.
StemMACS Cardiac Cultivation Medium XF is a complete and xeno-free cell culture media for consistent and reliable cultivation of cardiomyocytes derived from hPSCs. After directed differentiation using StemMACS CardioDiff Kit XF (# 130-125-289), PSC-derived cardiomyocytes can be maintained in culture in StemMACS Cardiac Cultivation Medium XF for more than 30 days. Additionally, StemMACS Cardiac Cultivation Medium XF facilitates a fast recovery after thawing of PSC-derived cardiomyocytes frozen in StemMACS Cryo-Brew (# 130-109-558). StemMACS Cardiac Cultivation Medium XF does not contain phenol red.

Applications

  • Cultivation of cardiomyocytes derived from human PSC lines

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