The PSC-Derived Cardiomyocyte Isolation Kit allows the magnetic separation of vital cardiomyocytes differentiated from human pluripotent stem cells. The procedure allows the enrichment of cardiomyocytes in less than an hour.

Data and images for PSC-Derived Cardiomyocyte Isolation Kit, human

Figures

Figure 1

Magnetically enriched PSC-derived cardiomyocytes show a typical morphology and sarcomeric structure. Troponin T stainings reveal the successful enrichment of vital cardiomyocytes (left: original fraction, right: enriched cardiomyocytes). Enriched cardiomyocytes can be further cultured on fibronectin-coated cell culture ware.
Before separation
After separation
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Figure 1

Magnetically enriched PSC-derived cardiomyocytes show a typical morphology and sarcomeric structure. Troponin T stainings reveal the successful enrichment of vital cardiomyocytes (left: original fraction, right: enriched cardiomyocytes). Enriched cardiomyocytes can be further cultured on fibronectin-coated cell culture ware.
View details

Figure 1

Magnetically enriched PSC-derived cardiomyocytes show a typical morphology and sarcomeric structure. Troponin T stainings reveal the successful enrichment of vital cardiomyocytes (left: original fraction, right: enriched cardiomyocytes). Enriched cardiomyocytes can be further cultured on fibronectin-coated cell culture ware.

Specifications for PSC-Derived Cardiomyocyte Isolation Kit, human

Overview

The PSC-Derived Cardiomyocyte Isolation Kit allows the magnetic separation of vital cardiomyocytes differentiated from human pluripotent stem cells. The procedure allows the enrichment of cardiomyocytes in less than an hour.

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Background information

Pure and well-characterized cardiomyocytes derived from human pluripotent stem cells (PSCs) are of high interest for cardiovascular disease modeling, drug safety studies, and development of cell replacement strategies. Although several protocols for cardiac differentiation of PSCs have been developed, there are still major limitations, including clone-to-clone and experiment-to-experiment variation in differentiation efficacy. To circumvent these limitations the PSC-Derived Cardiomycoyte Isolation Kit, human has been developed. It enables the purification of PSC-derived cardiomyocytes to high purities independent of the differentiation efficiency, the stem cell clone, the differentiation time point and differentiation protocol. The isolation of PSC-derived cardiomyocytes has successfully been tested for various differentiation efficiencies and time points of differentiation.

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