The Naive CD4
+
T Cell Isolation Kit II was developed for the isolation of untouched naive CD4
+
T cells from human peripheral blood.

Data and images for
Naive CD4
+
T Cell Isolation Kit II
, human

Figures

Figure 1

Isolation of untouched naive CD4
+
T helper cells from PBMCs using the Naive CD4
+
T Cell Isolation Kit II, an LS Column, and a MidiMACS™ Separator. Cells were stained with CD4 and CD45RA antibodies.
Unseparated fraction
Isolated untouched naive CD4
+
T cells
View details

Figure 1

Isolation of untouched naive CD4
+
T helper cells from PBMCs using the Naive CD4
+
T Cell Isolation Kit II, an LS Column, and a MidiMACS™ Separator. Cells were stained with CD4 and CD45RA antibodies.
View details

Figure 1

Isolation of untouched naive CD4
+
T helper cells from PBMCs using the Naive CD4
+
T Cell Isolation Kit II, an LS Column, and a MidiMACS™ Separator. Cells were stained with CD4 and CD45RA antibodies.

Specifications for
Naive CD4
+
T Cell Isolation Kit II
, human

Overview

The Naive CD4
+
T Cell Isolation Kit II was developed for the isolation of untouched naive CD4
+
T cells from human peripheral blood.

Detailed product information

Detailed separation procedure

For magnetic depletion of memory CD4
+
T cells and non-CD4
+
T cells, PBMCs are incubated with a cocktail of biotinylated CD45RO, CD8, CD14, CD15, CD16, CD19, CD25, CD34, CD36, CD56, CD123, anti-TCRγ/δ, anti-HLA-DR, and CD235a (glycophorin A) antibodies. These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads for depletion.

Downstream applications

Naive CD4
+
T cells isolated with the Naive CD4
+
T Cell Isolation Kit II can be used when direct labeling of cell surface molecules could interfere with downstream applications, e.g., in studies on naive T helper cell activation and in functional studies of DCs.
1, 2
Untouched naive CD4
+
T cells can also be used for further isolation of specific T helper cell subsets.
For isolation of naive CD4
+
recent thymic emigrants the CD4
+
Recent Thymic Emigrant Isolation Kit, human is recommended.

Columns

LS, XS, or autoMACS
®
Columns.

Resources for
Naive CD4
+
T Cell Isolation Kit II
, human

References for
Naive CD4
+
T Cell Isolation Kit II
, human

Publications

  1. Shen, W. et al. (2005)
    Modulation of CD4 Tʜ cell differentiation by ganglioside GD1a
    in vitro
    .
    J. Immunol. 175: 4927-4934
  2. Fernandez-Sesma, A. et al. (2006) Influenza virus evades innate and adaptive immunity via the NS1 protein. J. Virol. 80: 6295-6304

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Naive CD4
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T Cell Isolation Kit II
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