The IL-2 Secretion Assay - Cell Enrichment and Detection Kit was developed for the sensitive detection as well as the enrichment of murine IL-2-secreting cells.

Data and images for Mouse IL-2 Secretion Assay – Cell Enrichment and Detection Kit (PE)

Figures

Figure 1

BALB/c mice were immunized and the spleen cells restimulated with HEL as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-2 using the Mouse IL-2 Secretion Assay - Cell Enrichment and Detection Kit.
In the stimulated sample, 3125 IL-2-secreting CD4
+
T cells were enriched per 10
6
CD4
+
T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 720 IL-2-secreting CD4
+
T cells were enriched per 10
6
CD4
+
T cells.
Stimulated sample
Before enrichment
After enrichment
View details

Figure 1

BALB/c mice were immunized and the spleen cells restimulated with HEL as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-2 using the Mouse IL-2 Secretion Assay - Cell Enrichment and Detection Kit.
In the stimulated sample, 3125 IL-2-secreting CD4
+
T cells were enriched per 10
6
CD4
+
T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 720 IL-2-secreting CD4
+
T cells were enriched per 10
6
CD4
+
T cells.
View details

Figure 1

BALB/c mice were immunized and the spleen cells restimulated with HEL as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-2 using the Mouse IL-2 Secretion Assay - Cell Enrichment and Detection Kit.
In the stimulated sample, 3125 IL-2-secreting CD4
+
T cells were enriched per 10
6
CD4
+
T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 720 IL-2-secreting CD4
+
T cells were enriched per 10
6
CD4
+
T cells.
Unstimulated control
Before enrichment
After enrichment
View details

Figure 1

BALB/c mice were immunized and the spleen cells restimulated with HEL as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-2 using the Mouse IL-2 Secretion Assay - Cell Enrichment and Detection Kit.
In the stimulated sample, 3125 IL-2-secreting CD4
+
T cells were enriched per 10
6
CD4
+
T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 720 IL-2-secreting CD4
+
T cells were enriched per 10
6
CD4
+
T cells.
View details

Figure 1

BALB/c mice were immunized and the spleen cells restimulated with HEL as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-2 using the Mouse IL-2 Secretion Assay - Cell Enrichment and Detection Kit.
In the stimulated sample, 3125 IL-2-secreting CD4
+
T cells were enriched per 10
6
CD4
+
T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 720 IL-2-secreting CD4
+
T cells were enriched per 10
6
CD4
+
T cells.

Figure 2

BALB/c mice were immunized i.p. with keyhole limpet hemocyanin (KLH) in incomplete Freund's adjuvant and pertussis toxin. On day 21 after immunization, mouse spleen cells were restimulated
in vitro
with KLH for 15 hours. The responding cells were stained for coexpression of cytokines using the Mouse IFN-γ Secretion Assay - Detection Kit (PE) and Mouse IL-2 Secretion Assay - Detection Kit (APC). The plots show IFN-γ- and IL-2- secreting cells gated on viable CD4
+
T cells on the stimulated sample as well as on the unstimulated control.
Stimulated sample
Unstimulated control
View details

Figure 2

BALB/c mice were immunized i.p. with keyhole limpet hemocyanin (KLH) in incomplete Freund's adjuvant and pertussis toxin. On day 21 after immunization, mouse spleen cells were restimulated
in vitro
with KLH for 15 hours. The responding cells were stained for coexpression of cytokines using the Mouse IFN-γ Secretion Assay - Detection Kit (PE) and Mouse IL-2 Secretion Assay - Detection Kit (APC). The plots show IFN-γ- and IL-2- secreting cells gated on viable CD4
+
T cells on the stimulated sample as well as on the unstimulated control.
View details

Figure 2

BALB/c mice were immunized i.p. with keyhole limpet hemocyanin (KLH) in incomplete Freund's adjuvant and pertussis toxin. On day 21 after immunization, mouse spleen cells were restimulated
in vitro
with KLH for 15 hours. The responding cells were stained for coexpression of cytokines using the Mouse IFN-γ Secretion Assay - Detection Kit (PE) and Mouse IL-2 Secretion Assay - Detection Kit (APC). The plots show IFN-γ- and IL-2- secreting cells gated on viable CD4
+
T cells on the stimulated sample as well as on the unstimulated control.

Specifications for Mouse IL-2 Secretion Assay – Cell Enrichment and Detection Kit (PE)

Overview

The IL-2 Secretion Assay - Cell Enrichment and Detection Kit was developed for the sensitive detection as well as the enrichment of murine IL-2-secreting cells.

Detailed product information

Background information

Interleukin-2 (IL-2) is a T cell-derived cytokine known to promote growth and differentiation of T cells and has pleiotropic effects on many other leukocytes. IL-2 is rapidly secreted upon activation by naive T helper cells and certain subsets of memory T cells.

Downstream applications

The Mouse IL-2 Secretion Assay was used to study the sequential production of cytokines by T helper lymphocytes.
1
Furthermore, it was used for isolation and analysis of IL-2-secreting NKT cells after stimulation with glycolipids.
2
The IL-2 Secretion Assay has also been used as a highly sensitive read-out system for peptide-specific T cells in TCR transgenic mice.
3
T cell suppression by CD4
+
CD25
+
regulatory T cells has been analyzed with the IL-2 Secretion Assay by coculturing responder T cells with regulatory T cells (distinguished by Thy1.1 and Thy1.2 expression) followed by cytokine detection and costaining with Thy1.1.
4

Columns

MS, LS, or autoMACS
®
Columns.

References for Mouse IL-2 Secretion Assay – Cell Enrichment and Detection Kit (PE)

Publications

  1. Assenmacher et al. (1998) Sequential production of IL-2, IFN-gamma and IL-10 by individual staphylococcal enterotoxin B-activated T helper lymphocytes. Eur. J. Immunol. 28: 1534-1543
  2. Yang, J. Q. et al. (2003) Immunoregulatory role of CD1d in the hydrocarbon oil-induced model of lupus nephritis. J. Immunol. 171: 2142-2153
  3. Sojka, D. K. et al. (2004)
    IL-2 secretion by CD4
    +
    T cells
    in vivo
    is rapid, transient, and influenced by TCR-specific competition.
    J. Immunol. 172: 6136-6143
  4. Sojka, D. K. et al. (2005) Early kinetic window of target T cell susceptibility to CD25+ regulatory T cell activity. J. Immunol. 175: 7274-7280

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