Name: MACSxpress® Whole Blood CD4 Memory T Cell Isolation Kit, human
Availability: InStock

Data and images
Specifications

Product data and images for
MACSxpress
^®
Whole Blood CD4 Memory T Cell Isolation Kit
, human

Figures

Figure 1

Untouched CD4

⁺

memory T cells were isolated from 30 mL of human EDTA-anticoagulated whole blood using the MACSxpress

^®

Whole Blood CD4

⁺

Memory T Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45RO-APC, CD4-PE, CD45RA-FITC, and CD45-VioBlue

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer 10. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence. CD45RA versus CD45RO expression is shown for gated CD4

⁺

cells.

Before separation	After separation
View details Figure 1 Untouched CD4 ⁺ memory T cells were isolated from 30 mL of human EDTA-anticoagulated whole blood using the MACSxpress ^® Whole Blood CD4 ⁺ Memory T Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45RO-APC, CD4-PE, CD45RA-FITC, and CD45-VioBlue ^® and analyzed by flow cytometry using the MACSQuant ^® Analyzer 10. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence. CD45RA versus CD45RO expression is shown for gated CD4 ⁺ cells.	View details Figure 1 Untouched CD4 ⁺ memory T cells were isolated from 30 mL of human EDTA-anticoagulated whole blood using the MACSxpress ^® Whole Blood CD4 ⁺ Memory T Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45RO-APC, CD4-PE, CD45RA-FITC, and CD45-VioBlue ^® and analyzed by flow cytometry using the MACSQuant ^® Analyzer 10. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence. CD45RA versus CD45RO expression is shown for gated CD4 ⁺ cells.
View details Figure 1 Untouched CD4 ⁺ memory T cells were isolated from 30 mL of human EDTA-anticoagulated whole blood using the MACSxpress ^® Whole Blood CD4 ⁺ Memory T Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45RO-APC, CD4-PE, CD45RA-FITC, and CD45-VioBlue ^® and analyzed by flow cytometry using the MACSQuant ^® Analyzer 10. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence. CD45RA versus CD45RO expression is shown for gated CD4 ⁺ cells.	View details Figure 1 Untouched CD4 ⁺ memory T cells were isolated from 30 mL of human EDTA-anticoagulated whole blood using the MACSxpress ^® Whole Blood CD4 ⁺ Memory T Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45RO-APC, CD4-PE, CD45RA-FITC, and CD45-VioBlue ^® and analyzed by flow cytometry using the MACSQuant ^® Analyzer 10. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence. CD45RA versus CD45RO expression is shown for gated CD4 ⁺ cells.

Specifications for
MACSxpress
^®
Whole Blood CD4 Memory T Cell Isolation Kit
, human

Overview

The MACSxpress

^®

Whole Blood CD4 Memory T Cell Isolation Kit has been developed for the fast isolation of untouched memory T cells from freshly drawn anticoagulated whole blood without density gradient centrifugation and red blood cell lysis. Sample volumes of up to 30 mL can be processed.

Detailed product information

Detailed separation procedure

With the new MACSxpress

^®

Technology, untouched human leukocyte subsets can be isolated from up to 30 mL of anticoagulated whole blood. Non-target cells are removed by immunomagnetic depletion using MACSxpress Beads. Simultaneously, erythrocytes are sedimented, yielding untouched target cells of high purity.

Downstream applications

CD4

⁺

memory T cells isolated with the MACSxpress

^®

Whole Blood CD4 Memory T Cell Isolation Kit can be used, e.g., for phenotyping, biomarker analysis and functional assays.

Data and images

Data and images for
MACSxpress
^®
Whole Blood CD4 Memory T Cell Isolation Kit
, human

Figures

Figure 1

Untouched CD4

⁺

memory T cells were isolated from 30 mL of human EDTA-anticoagulated whole blood using the MACSxpress

^®

Whole Blood CD4

⁺

Memory T Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45RO-APC, CD4-PE, CD45RA-FITC, and CD45-VioBlue

^®

and analyzed by flow cytometry using the MACSQuant

^®

⁺

cells.

Before separation	After separation
View details Figure 1 Untouched CD4 ⁺ memory T cells were isolated from 30 mL of human EDTA-anticoagulated whole blood using the MACSxpress ^® Whole Blood CD4 ⁺ Memory T Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45RO-APC, CD4-PE, CD45RA-FITC, and CD45-VioBlue ^® and analyzed by flow cytometry using the MACSQuant ^® Analyzer 10. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence. CD45RA versus CD45RO expression is shown for gated CD4 ⁺ cells.	View details Figure 1 Untouched CD4 ⁺ memory T cells were isolated from 30 mL of human EDTA-anticoagulated whole blood using the MACSxpress ^® Whole Blood CD4 ⁺ Memory T Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45RO-APC, CD4-PE, CD45RA-FITC, and CD45-VioBlue ^® and analyzed by flow cytometry using the MACSQuant ^® Analyzer 10. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence. CD45RA versus CD45RO expression is shown for gated CD4 ⁺ cells.
View details Figure 1 Untouched CD4 ⁺ memory T cells were isolated from 30 mL of human EDTA-anticoagulated whole blood using the MACSxpress ^® Whole Blood CD4 ⁺ Memory T Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45RO-APC, CD4-PE, CD45RA-FITC, and CD45-VioBlue ^® and analyzed by flow cytometry using the MACSQuant ^® Analyzer 10. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence. CD45RA versus CD45RO expression is shown for gated CD4 ⁺ cells.	View details Figure 1 Untouched CD4 ⁺ memory T cells were isolated from 30 mL of human EDTA-anticoagulated whole blood using the MACSxpress ^® Whole Blood CD4 ⁺ Memory T Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45RO-APC, CD4-PE, CD45RA-FITC, and CD45-VioBlue ^® and analyzed by flow cytometry using the MACSQuant ^® Analyzer 10. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence. CD45RA versus CD45RO expression is shown for gated CD4 ⁺ cells.

Specifications

Specifications for
MACSxpress
^®
Whole Blood CD4 Memory T Cell Isolation Kit
, human

Overview

The MACSxpress

^®

Detailed product information

Detailed separation procedure

With the new MACSxpress

^®

Downstream applications

CD4

⁺

memory T cells isolated with the MACSxpress

^®

Whole Blood CD4 Memory T Cell Isolation Kit can be used, e.g., for phenotyping, biomarker analysis and functional assays.

MACSxpress
^®
Whole Blood CD4 Memory T Cell Isolation Kit
, human

MACSxpress
^®
Whole Blood CD4 Memory T Cell Isolation Kit
, human

Product overview

Product data and images for
MACSxpress
^®
Whole Blood CD4 Memory T Cell Isolation Kit
, human

Figures

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Specifications for
MACSxpress
^®
Whole Blood CD4 Memory T Cell Isolation Kit
, human

Overview

Detailed product information

Detailed separation procedure

Downstream applications

Data and images

Data and images for
MACSxpress
^®
Whole Blood CD4 Memory T Cell Isolation Kit
, human

Figures

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Specifications

Specifications for
MACSxpress
^®
Whole Blood CD4 Memory T Cell Isolation Kit
, human

Overview

Detailed product information

Detailed separation procedure

Downstream applications

Related products for

MACSxpress
^®
Whole Blood CD4 Memory T Cell Isolation Kit
, human

9 products available | view all view less

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Careers

About Miltenyi Biotec

MACSxpress® Whole Blood CD4 Memory T Cell Isolation Kit, human

MACSxpress® Whole Blood CD4 Memory T Cell Isolation Kit, human

Product overview

Product data and images for MACSxpress® Whole Blood CD4 Memory T Cell Isolation Kit, human

Figures

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Specifications for MACSxpress® Whole Blood CD4 Memory T Cell Isolation Kit, human

Overview

Detailed product information

Detailed separation procedure

Downstream applications

Data and images for MACSxpress® Whole Blood CD4 Memory T Cell Isolation Kit, human

Figures

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Specifications for MACSxpress® Whole Blood CD4 Memory T Cell Isolation Kit, human

Overview

Detailed product information

Detailed separation procedure

Downstream applications

Related products for MACSxpress® Whole Blood CD4 Memory T Cell Isolation Kit, human

9 products available | view all view less

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CD45RO Antibody, anti-human

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Careers

About Miltenyi Biotec

MACSxpress
^®
Whole Blood CD4 Memory T Cell Isolation Kit
, human

MACSxpress
^®
Whole Blood CD4 Memory T Cell Isolation Kit
, human

Product data and images for
MACSxpress
^®
Whole Blood CD4 Memory T Cell Isolation Kit
, human

Specifications for
MACSxpress
^®
Whole Blood CD4 Memory T Cell Isolation Kit
, human

Data and images for
MACSxpress
^®
Whole Blood CD4 Memory T Cell Isolation Kit
, human

Specifications for
MACSxpress
^®
Whole Blood CD4 Memory T Cell Isolation Kit
, human

Related products for

MACSxpress
^®
Whole Blood CD4 Memory T Cell Isolation Kit
, human