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LPS activated mouse splenocytes were stained with CD80 antibodies conjugated to (A) FITC (B) PE (C) APC (D) PE-Vio770 and (E) Biotin, as well as with CD11c antibodies conjugated to FITC or PE and analyzed by flow cytometry using the MACSQuant® Analyzer. Cells labeled with CD80-Biotin were stained with Anti-Biotin-PE as well. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of CD80-PE-Vio770.
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