The MACS
®
Comp Bead Kits have been developed for optimal compensation of the fluorescence spillover from fluorochrome-conjugated antibodies.

Data and images for
MACS
®
Comp Bead Kits

Figures

Figure 1

The MACS Comp Beads – anti-mouse Igκ were labeled with antibodies conjugated to FITC and PE and analyzed. Gating was performed on single bead events (A). MACS Comp Beads are shown before (B) and after (C) compensation using the Compensation Multicolor program with the MACSQuant
®
Analyzer.
A:
B:
View details

Figure 1

The MACS Comp Beads – anti-mouse Igκ were labeled with antibodies conjugated to FITC and PE and analyzed. Gating was performed on single bead events (A). MACS Comp Beads are shown before (B) and after (C) compensation using the Compensation Multicolor program with the MACSQuant
®
Analyzer.
View details

Figure 1

The MACS Comp Beads – anti-mouse Igκ were labeled with antibodies conjugated to FITC and PE and analyzed. Gating was performed on single bead events (A). MACS Comp Beads are shown before (B) and after (C) compensation using the Compensation Multicolor program with the MACSQuant
®
Analyzer.
C:
View details

Figure 1

The MACS Comp Beads – anti-mouse Igκ were labeled with antibodies conjugated to FITC and PE and analyzed. Gating was performed on single bead events (A). MACS Comp Beads are shown before (B) and after (C) compensation using the Compensation Multicolor program with the MACSQuant
®
Analyzer.

Specifications for
MACS
®
Comp Bead Kits

Overview

The MACS
®
Comp Bead Kits have been developed for optimal compensation of the fluorescence spillover from fluorochrome-conjugated antibodies.

Detailed product information

Most small molecule and protein-based fluorochromes have broad emission spectra which partially overlap with the spectra of other fluorochromes in flow cytometric analysis. This overlap should be detected and corrected for proper analysis. After staining with fluorochrome-conjugated antibodies, the MACS Comp Bead Kits can be used for automated or manual compensation along with the MACS Comp Beads – blank for the control of the negative population.

Resources for
MACS
®
Comp Bead Kits

Documents and Protocols

App notes/customer reports

Flow cytometry: Compensation