The Lung Endothelial Cell Isolation Kit, rat enables the enrichment of endothelial cells from adult rat lung using MACS
®
Cell Separation technology.
The isolation is performed in a two-step procedure. The enriched lung endothelial cells are fully functional and can be used for various downstream applications.

Data and images for Lung Endothelial Cell Isolation Kit, rat

Figures

Figure 1

Endothelial cells were isolated from adult CD-1
®
rat lung by using the Lung Endothelial Cell Isolation Kit, one LD Column with MidiMACS™ Separator, and one MS Column with MiniMACS™ Separator. The cells were fluorescently stained with Labeling Check Reagent-VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
A:
B:
Before separation
Enriched lung endothelial cells
View details

Figure 1

Endothelial cells were isolated from adult CD-1
®
rat lung by using the Lung Endothelial Cell Isolation Kit, one LD Column with MidiMACS™ Separator, and one MS Column with MiniMACS™ Separator. The cells were fluorescently stained with Labeling Check Reagent-VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Endothelial cells were isolated from adult CD-1
®
rat lung by using the Lung Endothelial Cell Isolation Kit, one LD Column with MidiMACS™ Separator, and one MS Column with MiniMACS™ Separator. The cells were fluorescently stained with Labeling Check Reagent-VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Lung Endothelial Cell Isolation Kit, rat

Overview

The Lung Endothelial Cell Isolation Kit, rat enables the enrichment of endothelial cells from adult rat lung using MACS
®
Cell Separation technology.
The isolation is performed in a two-step procedure. The enriched lung endothelial cells are fully functional and can be used for various downstream applications.

Detailed product information

Background information

Endothelial cells form the inner layer of blood vessels play a key role in vascular development, homeostasis, and remodeling.
The Lung Endothelial Cell Isolation Kit, rat has been designed for the enrichment of endothelial single cell suspensions from adult rat lung.
The isolation of endothelial cells has successfully been tested using adult rat lung in combination with the Multi Tissue Dissociation Kit 2.

Columns

For the first magnetic separation (depletion): LD Columns. For the second magnetic separation (positive selection): MS Columns.

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Lung Endothelial Cell Isolation Kit, rat

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