The Liver Dissociation Kit has been developed for the gentle, rapid, and efficient generation of single-cell suspensions from mouse liver. It has been optimized for a high yield of non-parenchymal mouse liver cells, including liver sinusoidal endothelial cells (LSECs) and Kupffer cells, while preserving most cell surface epitopes. Dissociated cells can be cultured or isolated using MACS
®
Technology.

Specifications for Liver Dissociation Kit, mouse

Overview

The Liver Dissociation Kit has been developed for the gentle, rapid, and efficient generation of single-cell suspensions from mouse liver. It has been optimized for a high yield of non-parenchymal mouse liver cells, including liver sinusoidal endothelial cells (LSECs) and Kupffer cells, while preserving most cell surface epitopes. Dissociated cells can be cultured or isolated using MACS
®
Technology.

Detailed product information

For gentle and efficient dissociation, mouse liver is enzymatically digested using the components of the kit, while the gentleMACS™ Dissociators are used for the mechanical dissociation steps. For highest convenience, choose the gentleMACS Octo Dissociator with Heaters enabling on-instrument enzyme incubation.

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