The Indirect CD133 MicroBead Kit was developed for positive selection or depletion of CD133
+
cells, using a two-step procedure involving indirect magnetic labeling.
  • Hematopoietic stem cells can be isolated from peripheral blood, cord blood, bone marrow, or leukapheresis product.
  • Neural progenitor cells can be isolated from single-cell suspensions from primary neural tissues or cell lines.
  • ES and iPS cell–derived neural, endothelial, or hematopoietic progenitors can be isolated from differentiated ES or iPS cell cultures1.

Data and images for Indirect CD133 MicroBead Kit, human

Figures

Figure 1

Separation of non-mobilized PBMCs using CD133/1 (AC133)-Biotin, Anti-Biotin MicroBeads, MS Columns, and a MiniMACS™ Separator. The cells are fluorescently stained with CD34-FITC and CD133/2 (293C3)-PE. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence.
Unseparated fraction
CD133+ cells
View details

Figure 1

Separation of non-mobilized PBMCs using CD133/1 (AC133)-Biotin, Anti-Biotin MicroBeads, MS Columns, and a MiniMACS™ Separator. The cells are fluorescently stained with CD34-FITC and CD133/2 (293C3)-PE. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence.
View details

Figure 1

Separation of non-mobilized PBMCs using CD133/1 (AC133)-Biotin, Anti-Biotin MicroBeads, MS Columns, and a MiniMACS™ Separator. The cells are fluorescently stained with CD34-FITC and CD133/2 (293C3)-PE. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence.

Specifications for Indirect CD133 MicroBead Kit, human

Overview

The Indirect CD133 MicroBead Kit was developed for positive selection or depletion of CD133
+
cells, using a two-step procedure involving indirect magnetic labeling.
  • Hematopoietic stem cells can be isolated from peripheral blood, cord blood, bone marrow, or leukapheresis product.
  • Neural progenitor cells can be isolated from single-cell suspensions from primary neural tissues or cell lines.
  • ES and iPS cell–derived neural, endothelial, or hematopoietic progenitors can be isolated from differentiated ES or iPS cell cultures1.
  • Cancer stem cells can be isolated from single-cell suspensions from primary tumor tissue or cell lines.

Detailed product information

Background information

CD133, formerly known as AC133, recognizes epitope 1 of the CD133 antigen.
2,3
It is a marker that is frequently found on multipotent progenitor cells, including immature hematopoietic stem and progenitor cells. In the hematopoietic system, CD133 is expressed on a small portion of CD34
cells
4
as well as on a subset of CD34
bright
stem and progenitor cells in human fetal liver, bone marrow, cord blood, and peripheral blood
5
. CD133 has also been found to be expressed on circulating endothelial progenitor cells;
6,7
fetal neural stem cells;
8,9
other tissue-specific stem cells, such as renal
10
, prostate
11
, and corneal
12
stem cells; cancer stem cells from tumor tissues; as well as ES and iPS cell-derived cells.
There is a growing interest in CD133 antigen expressing stem cells from normal blood or bone marrow in the field of regenerative medicine, for example bone marrow-derived CD133
+
stem cells in cardiovascular
13-17
, liver, or peripheral artery diseases
18-20
.
The CD133 antibody included in the kit recognizes epitope CD133/1. For quality control staining of CD133-separated cells, the use of CD133/2 (293C3)-PE or -APC is recommended.

Downstream applications

Isolated from hematopoietic sources, CD133
+
cells can become adherent and are reported to become CD133-negative during culture.
21,22
These adherent cells can then in turn give rise to nonadherent CD133
+
cells that are able to differentiate to both hematopoietic and nonhematopoietic cell types.
23
CD133
+
cells have shown a capacity for tissue differentiation, including to neural lineages
24
. CD133
+
isolated from fetal liver
25
, umbilical cord blood
26
, bone marrow
27
, mobilized blood
28
, and skin
29
are capable of in vitro differentiation to neuronal cells as well as to astrocytes,
25,26,28
oligodendrocytes,
26,28
and glial cells.
26
CD133
+
cells isolated from human fetal brain
8,9,30-32
were able to form self-renewing neurospheres in vitro, and to differentiate into neurons
8,32
and glia
19,23
. When injected into mice, human CD133
+
cells differentiated into fully integrated neurones and glial cells
9,30
as well as astrocytes and endothelial cells
29
. The CD34
+
CD133
+
cell population, which includes CD34
+
CD38
cells, was shown to be capable of repopulating NOD/SCID mice
33
.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.

References for Indirect CD133 MicroBead Kit, human

Publications

  1. Galic, Z. et al. (2006) T lineage differentiation from human embryonic stem cells. Proc. Natl. Acad. Sci. U.S.A. 103: 11742-11747
  2. Yin, A. H. et al. (1997) AC133, a novel marker for human hematopoietic stem and progenitor cells. Blood 90: 5002-5012
  3. Piechaczek, C. (2001) CD133. J. Biol. Regul. Homeost. Agents 15: 101-102
  4. Gallacher, L. et al. (2000)
    Isolation and characterization of human CD34
    Lin
    and CD34
    +
    Lin
    hematopoietic stem cells using cell surface markers AC133 and CD7.
    Blood 95(ARVO Annual Meeting Abstract): 2813-2820
  5. Bühring, H. J. et al. (1999) Expression of novel surface antigens on early hematopoietic cells. Ann. N. Y. Acad. Sci. 872: 25-39
  6. Gehling, U. M. et al. (2000)
    In vitro
    differentiation of endothelial cells from AC133-positive progenitor cells.
    Blood 95: 3106-3112
  7. Peichev, M. et al. (2000)
    Expression of VEGFR-2 and AC133 by circulating human CD34
    +
    cells identifies a population of functional endothelial precursors.
    Blood 95: 952-958
  8. Uchida, N. et al. (2000) Direct isolation of human central nervous system stem cells. Proc. Natl. Acad. Sci. U.S.A. 97: 14720-14725
  9. Cummings, B. J. et al. (2005) Human neural stem cells differentiate and promote locometer recovery in spinal cord-injured mice. Proc. Natl. Acad. Sci. U.S.A. 102: 14069-14074
  10. Bussolati, B. et al. (2005) Isolation of renal progenitor cells from adult human kidney. Am. J. Pathol. 166: 545-555
  11. Richardson, G. et al. (2004) CD133, a novel marker for human prostatic epithelial stem cells. J. Cell. Sci. 117: 3539-3545
  12. Thill, M. et al. (2004)
    Identification of a population of CD133
    +
    precursor cells in the stroma of human cornea.
    Invest. Ophthalmol. Vis. Sci. 45: 3519
  13. Stamm, C. et al. (2003) Autologous bone-marrow stem-cell transplantation for myocardial regeneration. Lancet 4, 361(9351): 45-46
  14. Stamm, C. et al. (2007)
    Intramyocardial delivery of CD133
    +
    bone marrow cells and coronary artery bypass grafting for chronic ischemic heart disease: safety and efficacy studies.
    J. Thorac. Cardiovasc. Surg. 133: 717-725
  15. Klein (2007) Eur. Cardiovasc. Dis. 1: 123-125
  16. Klein, H. M. et al. (2007)
    Intramyocardial implantation of CD133
    +
    stem cells improved cardiac function without bypass surgery.
    Heart Surg. Forum 10: E66-69
  17. Bartunek et al. (2005) Circulation 30: 178-183
  18. am Esch, J. S. 2nd et al. (2005)
    Portal application of autologous CD133
    +
    bone marrow cells to the liver: a novel concept to support hepatic regeneration.
    Stem Cells 23(4): 463-470
  19. Fürst, G. et al. (2007)
    Portal vein embolization and autologous CD133
    +
    bone marrow stem cells for liver regeneration: initial experience.
    J. Immunother. 243(1): 171-179
  20. Cañizo et al. (2007)
    Peripheral endothelial progenitor cells (CD133
    +
    ) for therapeutic vasculogenesis in a patient with critical limb ischemia. One year follow-up.
    Cytotherapy 9(1): 99-102
  21. Kuçi et al. (2003)
    Identification of a novel class of human adherent CD34
    stem cells that give rise to SCID-repopulating cells.
    Blood 101: 869-876
  22. Kuçi et al. (2008)
    Efficient
    in vitro
    generation of adult multipotent cells from mobilized peripheral blood CD133
    +
    cells.
    Cell Prolif. 41: 12-27
  23. Kuçi et al. (2003) MACS&more 7(1): 6-8
  24. Kuçi et al. (2004) 2nd Intl. Meeting, Stem Cell Network, North-Rhine Westphalia. : abstract
  25. Hao, H. N. et al. (2003)
    Fetal human hematopoietic stem cells can differentiate sequentially into neural stem cells and then astrocytes
    in vitro
    .
    J. Hematother. Stem Cell Res. 12: 23-32
  26. Jang, Y. K. et al. (2004) Retinoic acid-mediated induction of neurons and glial cells from human umbilical cord-derived hematopoietic stem cells. J. Neurosci. Res. 75: 573-584
  27. Padovan et al. (2003) Expression of neuronal markers in differentiated marrow stromal cells and CD133+ stem-like cells. Cell Transplant 12: 839-848
  28. Piechaczek, C. et al. (2002)
    Differentiation of adult CD133
    +
    cells isolated from peripheral blood into cells with a neural phenotype.
    Stem cell research (customer reports published by Miltenyi Biotec) : 2-3
  29. Belicchi, M. et al. (2004) Human skin-derived stem cells migrate throughout forebrain and differentiate into astrocytes after injection into adult mouse brain. J. Neurosci. Res. 77: 475-486
  30. Tamaki, S. et al. (2002) Engraftment of sorted/expanded human central nervous system stem cells from fetal brain. J. Neurol. Res. 69: 976-986
  31. Kelly, S. et al. (2004) Transplanted human fetal neural stem cells survive, migrate, and differentiate in ischemic rat cerebral cortex. Proc. Natl. Acad. Sci. U.S.A. 101: 11839-11844
  32. Yu, S. et al. (2004)
    Isolation and characterization of the CD133
    +
    precursors from the ventricular zone of human fetal brain by magnetic affinity cell sorting.
    Biotechnol. Lett. 26: 1131-1136
  33. de Wynter, E. A. et al. (1998)
    CD34
    +
    AC133
    +
    cells isolated from cord blood are highly enriched in long-term culture-initiating cells, NOD/SCID-repopulating cells and dendritic cell progenitors.
    Stem Cells 16: 387-396

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