The IL-2 Secretion Assay - Cell Enrichment and Detection Kit was developed for the sensitive detection as well as the enrichment of human IL-2-secreting cells.

Data and images for IL-2 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Figures

Figure 1

PBMCs from a CMV
+
donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-2 using the IL-2 Secretion Assay - Cell Enrichment and Detection Kit.
In the stimulated sample, 2047 IL-2-secreting CD4
+
T cells were enriched per 10
6
CD4
+
T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only one IL-2-secreting CD4
+
T cell was detected per 10
6
CD4
+
T cells after enrichment.
Stimulated sample
Before enrichment
After enrichment
View details

Figure 1

PBMCs from a CMV
+
donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-2 using the IL-2 Secretion Assay - Cell Enrichment and Detection Kit.
In the stimulated sample, 2047 IL-2-secreting CD4
+
T cells were enriched per 10
6
CD4
+
T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only one IL-2-secreting CD4
+
T cell was detected per 10
6
CD4
+
T cells after enrichment.
View details

Figure 1

PBMCs from a CMV
+
donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-2 using the IL-2 Secretion Assay - Cell Enrichment and Detection Kit.
In the stimulated sample, 2047 IL-2-secreting CD4
+
T cells were enriched per 10
6
CD4
+
T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only one IL-2-secreting CD4
+
T cell was detected per 10
6
CD4
+
T cells after enrichment.
Unstimulated control
Before enrichment
After enrichment
View details

Figure 1

PBMCs from a CMV
+
donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-2 using the IL-2 Secretion Assay - Cell Enrichment and Detection Kit.
In the stimulated sample, 2047 IL-2-secreting CD4
+
T cells were enriched per 10
6
CD4
+
T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only one IL-2-secreting CD4
+
T cell was detected per 10
6
CD4
+
T cells after enrichment.
View details

Figure 1

PBMCs from a CMV
+
donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-2 using the IL-2 Secretion Assay - Cell Enrichment and Detection Kit.
In the stimulated sample, 2047 IL-2-secreting CD4
+
T cells were enriched per 10
6
CD4
+
T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only one IL-2-secreting CD4
+
T cell was detected per 10
6
CD4
+
T cells after enrichment.

Specifications for IL-2 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Overview

The IL-2 Secretion Assay - Cell Enrichment and Detection Kit was developed for the sensitive detection as well as the enrichment of human IL-2-secreting cells.

Detailed product information

Background information

Interleukin-2 (IL-2) is a T cell-derived cytokine known to promote growth and differentiation of T cells and to have pleiotropic effects on many other leukocytes. Upon activation, naive T helper cells and certain subsets of memory T cells rapidly secrete IL-2.

Downstream applications

The IL-2 Secretion Assay has been developed for detection and isolation of IL-2-secreting T cells after antigen-specific stimulation. IL-2 Secretion Assay has been used to analyze IL-2-secreting CMV-specific CD4
+
T cells after stimulation with CMV lysate.

Columns

MS, LS, or autoMACS
®
Columns.

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