The IL-17 Secretion Assay – Cell Enrichment and Detection Kit was developed for the sensitive detection and enrichment of viable human IL-17–secreting leukocytes from PBMCs.
The used Anti-PE MicroBeads UltraPure enhances sensitivity and minimizes background of debris and dead cells.

Data and images for IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Figures

Figure 1

PBMCs were stimulated with CytoStim for 4 hours or left untreated. Both samples were treated with a CD40 antibody to allow the surface detection of CD154. Cells were enriched by using the IL-17 Secretion Assay – Cell Enrichment and Detection Kit. The dot plots show stimulated and unstimulated cells before and after separation. Secretion of IL-17 by CD154
+
cells is demonstrated. Data were gated on viable CD4
+
cells.
Restimulated sample
Before enrichment
After enrichment
View details

Figure 1

PBMCs were stimulated with CytoStim for 4 hours or left untreated. Both samples were treated with a CD40 antibody to allow the surface detection of CD154. Cells were enriched by using the IL-17 Secretion Assay – Cell Enrichment and Detection Kit. The dot plots show stimulated and unstimulated cells before and after separation. Secretion of IL-17 by CD154
+
cells is demonstrated. Data were gated on viable CD4
+
cells.
View details

Figure 1

PBMCs were stimulated with CytoStim for 4 hours or left untreated. Both samples were treated with a CD40 antibody to allow the surface detection of CD154. Cells were enriched by using the IL-17 Secretion Assay – Cell Enrichment and Detection Kit. The dot plots show stimulated and unstimulated cells before and after separation. Secretion of IL-17 by CD154
+
cells is demonstrated. Data were gated on viable CD4
+
cells.
Without restimulation
Before enrichment
After enrichment
View details

Figure 1

PBMCs were stimulated with CytoStim for 4 hours or left untreated. Both samples were treated with a CD40 antibody to allow the surface detection of CD154. Cells were enriched by using the IL-17 Secretion Assay – Cell Enrichment and Detection Kit. The dot plots show stimulated and unstimulated cells before and after separation. Secretion of IL-17 by CD154
+
cells is demonstrated. Data were gated on viable CD4
+
cells.
View details

Figure 1

PBMCs were stimulated with CytoStim for 4 hours or left untreated. Both samples were treated with a CD40 antibody to allow the surface detection of CD154. Cells were enriched by using the IL-17 Secretion Assay – Cell Enrichment and Detection Kit. The dot plots show stimulated and unstimulated cells before and after separation. Secretion of IL-17 by CD154
+
cells is demonstrated. Data were gated on viable CD4
+
cells.

Specifications for IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Overview

The IL-17 Secretion Assay – Cell Enrichment and Detection Kit was developed for the sensitive detection and enrichment of viable human IL-17–secreting leukocytes from PBMCs.
The used Anti-PE MicroBeads UltraPure enhances sensitivity and minimizes background of debris and dead cells.

Detailed product information

Background information

Interleukin 17 (IL-17) is produced by CD4
+
T helper cells, a T cell subset termed Th17, which also secrete IL-17F and IL-22 and express the NK cell marker CD161
1
. IL-17 secretion has also been described for other cell types, such as CD8
+
memory T cells.
Th17 cells are involved in the recruitment of neutrophils to control early stages of infections by a number of pathogens, such as extracellular bacteria and fungi. IL-17 and Th17 cells play an important role in immune-mediated inflammatory diseases, such as rheumatoid arthritis, psoriasis, multiple sclerosis, asthma, and inflammatory bowel disease.

Columns

MS, LS, or autoMACS
®
Columns.

References for IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Publications

  1. Cosmi, L. et al. (2008)
    Human interleukin 17–producing cells originate from a CD161
    +
    CD4
    +
    T cell precursor.
    J. Exp. Med. 205: 1903-1916

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