Clone:
REA919
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC
Alternative names:
Ikaros family zinc finger protein 1, Lyf1, Zfpn1a1, Znfn1a1, Ikzf1

Extended validation for Ikaros Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA919
4E9-
16B5C71-
2A9/ikaros++
Cells were incubated with an excess of purified unconjugated Ikaros (REA919) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for Ikaros. Splenocytes from C57BL/6 mice were stained with a suitable counterstaining followed by a fixation and permeabilization step using the FoxP3 Staining Buffer Set. Cells were then stained with Ikaros antibodies. As a control, Ikaros antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Ikaros. Splenocytes from C57BL/6 mice were stained with a suitable counterstaining followed by a fixation and permeabilization step using the FoxP3 Staining Buffer Set. Cells were then stained with Ikaros antibodies. As a control, Ikaros antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Ikaros. Splenocytes from C57BL/6 mice were stained with a suitable counterstaining followed by a fixation and permeabilization step using the FoxP3 Staining Buffer Set. Cells were then stained with Ikaros antibodies. As a control, Ikaros antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Ikaros. Splenocytes from C57BL/6 mice were stained with a suitable counterstaining followed by a fixation and permeabilization step using the FoxP3 Staining Buffer Set. Cells were then stained with Ikaros antibodies. As a control, Ikaros antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Ikaros. Splenocytes from C57BL/6 mice were stained with a suitable counterstaining followed by a fixation and permeabilization step using the FoxP3 Staining Buffer Set. Cells were then stained with Ikaros antibodies. As a control, Ikaros antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for Ikaros. Splenocytes from C57BL/6 mice were stained with a suitable counterstaining followed by a fixation and permeabilization step using the FoxP3 Staining Buffer Set. Cells were then stained with Ikaros antibodies. As a control, Ikaros antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for Ikaros Antibody, anti-mouse, REAfinity™

Overview

Clone REA919 recognizes the mouse Ikaros protein, also known as DNA-binding protein Ikaros, Ikaros family zinc finger protein 1, or IKZF1. Ikaros is a transcription regulator of hematopoietic cell differentiation of lymphocytes, B cells, and T cells, and functions as a major tumor suppressor involved in human B cell acute lymphoblastic leukemia. In association with other Ikaros-family members, e.g. Aiolos and Helios, Ikaros forms heterodimers, that are important for normal progression to a mature and immunocompetent state. Ikaros is found in the nucleus to a higher extent in granulocytes, B cells, T cells, and NK cells, and in lower levels in erythroblasts, megakaryocytes, and monocytes.
Additional information: Clone REA919 displays negligible binding to Fc receptors.

Alternative names

Ikaros family zinc finger protein 1, Lyf1, Zfpn1a1, Znfn1a1, Ikzf1

Detailed product information

Technical specifications

CloneREA919
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenIkaros
Alternative names of antigenIkaros family zinc finger protein 1, Lyf1, Zfpn1a1, Znfn1a1, Ikzf1
Molecular mass of antigen [kDa]57
Distribution of antigenB cells, T cells, megakaryocytes, monocytes, NK cells, granulocytes
Entrez Gene ID22778
RRIDAB_2726976, AB_2727034, AB_2726977, AB_2727035, AB_2726978, AB_2727033

References for Ikaros Antibody, anti-mouse, REAfinity™

Publications

  1. Morgan, B. et al. (1997) Aiolos, a lymphoid restricted transcription factor that interacts with Ikaros to regulate lymphocyte differentiation. EMBO J. 16(8): 2004-2013
  2. Watkins, N. A. et al. (2009) A HaemAtlas: characterizing gene expression in differentiated human blood cells. Blood 113(19)
  3. Kastner, P. et al. (2011) Role of Ikaros in T-cell acute lymphoblastic leukemia. J. Biol. Chem. 2(6): 108-114

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