Clone:
REA1049
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC

Extended validation for IgE Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA1049
MHE-18+
Ige21-
G7-26+
MB10-5C4++
Cells were incubated with an excess of purified unconjugated IgE (REA1049) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for Anti-IgE. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-IgE antibodies and with a suitable counterstaining. As a control, Anti-IgE antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-IgE. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-IgE antibodies and with a suitable counterstaining. As a control, Anti-IgE antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-IgE. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-IgE antibodies and with a suitable counterstaining. As a control, Anti-IgE antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-IgE. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-IgE antibodies and with a suitable counterstaining. As a control, Anti-IgE antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for Anti-IgE. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-IgE antibodies and with a suitable counterstaining. As a control, Anti-IgE antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using IgE (REA1049). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using IgE (REA1049). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using IgE (REA1049). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for IgE Antibody, anti-human, REAfinity™

Overview

Clone REA1049 recognizes the IgE isotype of human immunoglobulins. IgE is the shortest-lived immunoglobulin with a half-life of two days in serum. IgE plays an important role in allergy. It is especially associated with type 1 hypersensitivity, where IgE shows high-affinity binding to Fc receptors expressed on the surface of mast cells. Antigen binding to Fc receptor–bound IgE upon re-exposure to specific allergens results in degranulation and the release of a variety of mediators, such as histamine and cytokines.
IgE has two main receptors: the high-affinity receptor FcεRI, which is expressed only on mast cells and basophils, and the low-affinity receptor FcεRII (CD23), which is expressed on B cells.
Additional information: Clone REA1049 displays negligible binding to Fc receptors.

Detailed product information

Technical specifications

CloneREA1049
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, non-human primate
Cross-reactivity
cynomolgus monkey (
Macaca fascicularis
)
AntigenIgE
Distribution of antigenB cells, mast cells, basophils
Entrez Gene ID3497
RRIDAB_2733670, AB_2733862, AB_2733863, AB_2733423, AB_2733424, AB_2733960, AB_2733961, AB_2734100, AB_2734101, AB_2732996, AB_2732997, AB_2733262, AB_2733263, AB_2733053, AB_2733054, AB_2734037, AB_2734038, AB_2733548, AB_2733549, AB_2733669

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