Clone:
REAL280
Type of antibody:
Releasable fluorochromes, Primary antibodies, Recombinant antibodies
Applications:
MICS, IF, IHC
Alternative names:
MHC class II HLA-DR, MHC class II HLA-DP, MHC class II HLA-DQ

Specifications for HLA-DR, DP, DQ Antibody, anti-human, REAdye_lease™

Overview

Clone REAL280 is an antibody fragment derived from the full HLA-DR, DP, DQ antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAdye_lease Complex to bind markers with high avidity.
Clone REAL280 recognizes all major histocompatibility Class (MHC) class II HLA-DR, DP and most DQ antigens. It is expressed on several nucleated cells, e.g. B cells, interdigitating reticulum cells, Langerhans cells, activated T cells, and macrophages. MHC class II molecules play an important role in the immune system. They are essential in the defense against infection and are a main consideration in transplantation medicine. In addition to presenting antigenic peptides from predominantly extracellular sources to CD4
+
T cells, MHC class II molecules also mediate the thymic selection of helper T cells. MHC class II molecules consist of an α and β chain and are transported to endosomal-lysosomal compartments by the invariant chain. In humans, MHC class II molecules are encoded by three different loci, HLA-DR, -DQ, and -DP, which display 70% similarity to each other. Despite the essential function of MHC class II molecules in immune defense against pathogens, some alleles are frequently linked to immune diseases.
For removal of REAdye_lease fluorochromes for optional relabeling with different fluorochrome-conjugated REAdye_lease antibodies use the REAlease Support Kit (130-120-675).

Alternative names

MHC class II HLA-DR, MHC class II HLA-DP, MHC class II HLA-DQ

Detailed product information

Technical specifications

CloneREAL280
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable fluorochromes, Primary antibodies, Recombinant antibodies
Specieshuman
AntigenHLA-DR, DP, DQ
Alternative names of antigenMHC class II HLA-DR, MHC class II HLA-DP, MHC class II HLA-DQ
Distribution of antigenT cells, CD4+ T cells, spleen

References for HLA-DR, DP, DQ Antibody, anti-human, REAdye_lease™

Publications

  1. Nishimura, Y. et al. (1998) Peptide-based molecular analyses of HLA class II-associated susceptibility to autoimmune diseases. Int. Rev. Immunol. 17(5–6): 229-262
  2. Jones, E. Y. et al. (2006) MHC class II proteins and disease: a structural perspective. Nat. Rev. Immunol. 6(4): 271-282
  3. van Lith, M. et al. (2010) HLA-DP, HLA-DQ, and HLA-DR have different requirements for invariant chain and HLA-DM. J. Biol. Chem. 285(52): 40800-40808