Clone:
REAL550
Type of antibody:
Releasable fluorochromes, Primary antibodies, Recombinant antibodies
Applications:
MICS, IHC, IF
Alternative names:
HLA-DRA, HLA-DRB1, HLA-DRB3, HLA-DRB4, HLA-DRB5

Specifications for HLA-DR Antibody, anti-human, REAdye_lease™

Overview

Clone REAL550 is an antibody fragment derived from the full HLA-DR antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAdye_lease Complex to bind markers with high avidity.
Clone REAL550 recognizes the human HLA-DR. It is constitutively expressed on professional antigen-presenting cells like dendritic cells, B cells, and monocytes/ macrophages. Its expression is further up-regulated upon activation. On T cells, NK cells, hematopoietic precursor cells, and some epithelial cells the expression of HLA-DR is induced by cell activation.
For removal of REAdye_lease fluorochromes for optional relabeling with different fluorochrome-conjugated REAdye_lease antibodies use the REAlease Support Kit (130-120-675).

Alternative names

HLA-DRA, HLA-DRB1, HLA-DRB3, HLA-DRB4, HLA-DRB5

Detailed product information

Technical specifications

CloneREAL550
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable fluorochromes, Primary antibodies, Recombinant antibodies
Specieshuman
AntigenHLA-DR
Alternative names of antigenHLA-DRA, HLA-DRB1, HLA-DRB3, HLA-DRB4, HLA-DRB5
Distribution of antigendendritic cells, monocytes, macrophages, NK cells, epithelial cells, other, tonsil
RRIDAB_2857572

References for HLA-DR Antibody, anti-human, REAdye_lease™

Publications

  1. Edwards, J. A. et al. (1986) Differential expression of HLA class II antigens in fetal human spleen: relationship of HLA-DP, DQ, and DR to immunoglobulin expression. J. Immunol. 137(2): 490-497
  2. Brodsky, F. M. (1984) A matrix approach to human class II histocompatibility antigens: reactions of four monoclonal antibodies with the products of nine haplotypes. Immunogenetics 19(3): 179-194
  3. Li, C. W. et al. (2017) Flexible peptide recognition by HLA-DR triggers specific autoimmune T-cell responses in autoimmune thyroiditis and diabetes. J. Autoimmun. 76: 1-9