The GM-CSF Secretion Assay - Cell Enrichment and Detection Kit was developed for enrichment and sensitive detection of human GM-CSF-secreting cells.

Data and images for GM-CSF Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Figures

Figure 1

PBMCs were stimulated with PMA and Ionomycin or left untreated. Cells were stained and isolated according to secretion of GM-CSF using the GM-CSF Secretion Assay - Cell Enrichment and Detection Kit (PE). The cells were counterstained with CD4-APC. B cells, monocytes and dead cells were excluded according to CD20-PerCP-Vio700, CD14-PerCP-Vio700 and PI-staining respectively.
Stimulated sample
Stimulated sample
After enrichment
View details

Figure 1

PBMCs were stimulated with PMA and Ionomycin or left untreated. Cells were stained and isolated according to secretion of GM-CSF using the GM-CSF Secretion Assay - Cell Enrichment and Detection Kit (PE). The cells were counterstained with CD4-APC. B cells, monocytes and dead cells were excluded according to CD20-PerCP-Vio700, CD14-PerCP-Vio700 and PI-staining respectively.
View details

Figure 1

PBMCs were stimulated with PMA and Ionomycin or left untreated. Cells were stained and isolated according to secretion of GM-CSF using the GM-CSF Secretion Assay - Cell Enrichment and Detection Kit (PE). The cells were counterstained with CD4-APC. B cells, monocytes and dead cells were excluded according to CD20-PerCP-Vio700, CD14-PerCP-Vio700 and PI-staining respectively.
Unstimulated control
Unstimulated control
After enrichment
View details

Figure 1

PBMCs were stimulated with PMA and Ionomycin or left untreated. Cells were stained and isolated according to secretion of GM-CSF using the GM-CSF Secretion Assay - Cell Enrichment and Detection Kit (PE). The cells were counterstained with CD4-APC. B cells, monocytes and dead cells were excluded according to CD20-PerCP-Vio700, CD14-PerCP-Vio700 and PI-staining respectively.
View details

Figure 1

PBMCs were stimulated with PMA and Ionomycin or left untreated. Cells were stained and isolated according to secretion of GM-CSF using the GM-CSF Secretion Assay - Cell Enrichment and Detection Kit (PE). The cells were counterstained with CD4-APC. B cells, monocytes and dead cells were excluded according to CD20-PerCP-Vio700, CD14-PerCP-Vio700 and PI-staining respectively.

Specifications for GM-CSF Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Overview

The GM-CSF Secretion Assay - Cell Enrichment and Detection Kit was developed for enrichment and sensitive detection of human GM-CSF-secreting cells.

Detailed product information

Background information

Granulocyte macrophage colony-stimulating factor (GM-CSF) is a hematopoietic growth factor that is essential for proliferation and development of granulocyte and monocyte/macrophage progenitors. It also functions as a growth factor for erythroid and megakaryocytic precursor cells in conjunction with erythropoietin. GM-CSF is secreted by various cell types including T cells, macrophages, endothelial cells, and fibroblasts in response to inflammatory stimuli and cytokines. In addition, GM-CSF strongly chemoattracts neutrophils and eosinophils and enhances the effector functions of neutrophils and macrophages.

Downstream applications

  • Enrichment and detection of viable GM-CSF–secreting leukocytes.
  • Enrichment and detection of viable GM-CSF–secreting leukocytes from whole blood.
  • Enrichment and detection of GM-CSF–secreting, antigen-specific T cells for enumeration and phenotypic characterization as well as for expansion and functional characterization.
  • Monitoring and analysis of very low frequent antigen-specific T cell immunity in autoimmunity.
  • Isolation and expansion of antigen-specific T cells.
  • Enrichment and analysis of GM-CSF–secreting cells for determination of functional antigens in disease and for T cell receptor (TCR) epitope mapping.
  • Analysis or cloning of TCR repertoire of antigen-specific T cells.

Columns

MS, LS, or autoMACS
®
Columns.

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