Clone:
REA174
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC, IF, IHC, MC
Alternative names:
HDR, HDRS, jal

Extended validation for GATA3 Antibody, anti-human/mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA174
L50-823-
TWAJ++
16E10A23+
Cells were incubated with an excess of purified unconjugated GATA3 (REA174) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for GATA3. Jurkat cells were fixed and permeabilized using the Transcription Factor Staining Buffer Set followed by intracellular staining with GATA3 antibodies and plotted against the side scatter. As a control, GATA3 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for GATA3. Jurkat cells were fixed and permeabilized using the Transcription Factor Staining Buffer Set followed by intracellular staining with GATA3 antibodies and plotted against the side scatter. As a control, GATA3 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for GATA3. Jurkat cells were fixed and permeabilized using the Transcription Factor Staining Buffer Set followed by intracellular staining with GATA3 antibodies and plotted against the side scatter. As a control, GATA3 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for GATA3. Jurkat cells were fixed and permeabilized using the Transcription Factor Staining Buffer Set followed by intracellular staining with GATA3 antibodies and plotted against the side scatter. As a control, GATA3 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for GATA3. Jurkat cells were fixed and permeabilized using the Transcription Factor Staining Buffer Set followed by intracellular staining with GATA3 antibodies and plotted against the side scatter. As a control, GATA3 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for GATA3. Jurkat cells were fixed and permeabilized using the Transcription Factor Staining Buffer Set followed by intracellular staining with GATA3 antibodies and plotted against the side scatter. As a control, GATA3 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals. The recommended titers of respective antibodies from different suppliers were used.

Specifications for GATA3 Antibody, anti-human/mouse, REAfinity™

Overview

Clone REA174 recognizes GATA3, a member of the GATA family of transcription factors. Like other members of the family, GATA3 contain zinc finger motifs involved in recognizing the canonical GATA DNA sequence. GATA3 has two trans-activating domains and two zinc finger domains, each followed by a conserved basic region. Expression of GATA3 is found in developing and mature T cells, natural killer (NK) cells, subset of NKT cells, and endothelial cells. Primary function attributed to GATA3 is to regulate the T helper 2 (Tʜ2) cell differentiation. However, role of GATA3 is also recognized in early T cell commitment and CD4
+
T cell development .
Additional information: Clone REA174 displays negligible binding to Fc receptors.      

Alternative names

HDR, HDRS, jal

Detailed product information

Technical specifications

CloneREA174
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, mouse
AntigenGATA3
Alternative names of antigenHDR, HDRS, jal
Molecular mass of antigen [kDa]48
Distribution of antigenT cells
Entrez Gene ID2625
RRIDAB_2751982, AB_2857654, AB_2857629, AB_2857663, AB_2857641, AB_2651829, AB_2651827, AB_2651828, AB_2752012

Reviews for GATA3 Antibody, anti-human/mouse, REAfinity™

Review of Recombinant GATA3 Antibody from Miltenyi

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  • 5

Anti-GATA3-PE-Vio615, human and mouse (130-109-115)

Bright, very good resolution. Would recommend.

Review of Recombinant GATA3 Antibody from Miltenyi

  • 1
  • 2
  • 3
  • 4
  • 5

Anti-GATA3-PE-Vio615, human and mouse (130-109-161)

Bright, very good resolution. Would recommend.

References for GATA3 Antibody, anti-human/mouse, REAfinity™

Publications

  1. Ho, I. et al. (2007) GATA-3 - not just for Tʜ2 cells anymore. Cell. Mol. Immunol. 4(1): 15-29
  2. Zaiss, D. M. et al. (2017) Epidermal growth factor receptor expression licenses type-2 helper T cells to function in a T cell receptor-independent fashion. Immunity 47(4): 710-722
  3. Ting, C. N. et al. (1996) Transcription factor GATA-3 is required for development of the T-cell lineage. Nature 284(6608): 474-478
  4. Joulin, V. et al. (1991) A T-cell specific TCR delta DNA binding protein is a member of the human GATA family. EMBO J. 10: 1809-1816
  5. Qian, L. et al. (2014) Alkylglycerols modulate the proliferation and differentiation of non-specific agonist and specific antigen-stimulated splenic lymphocytes. PLoS One 9(4): e96207
  6. Martinelli, G. et al. (2018) Immunosuppressive Treg cells acquire the phenotype of effector-T cells in chronic lymphocytic leukemia patients. J. Transl. Med. 16(1): 172

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