Clone:
REA758
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC, MC
Alternative names:
FCER1A, Fce1a, FcERI

Extended validation for FcεRIα Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA758
CRA1++
Cells were incubated with an excess of purified unconjugated FcεRIα (REA758) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for Anti-FCeRIa. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-FCeRIa antibodies and with a suitable counterstaining. As a control, Anti-FCeRIa antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-FCeRIa. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-FCeRIa antibodies and with a suitable counterstaining. As a control, Anti-FCeRIa antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-FCeRIa. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-FCeRIa antibodies and with a suitable counterstaining. As a control, Anti-FCeRIa antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-FCeRIa. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-FCeRIa antibodies and with a suitable counterstaining. As a control, Anti-FCeRIa antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for Anti-FCeRIa. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-FCeRIa antibodies and with a suitable counterstaining. As a control, Anti-FCeRIa antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using FcεRIα (REA758). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using FcεRIα (REA758). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using FcεRIα (REA758). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for FcεRIα Antibody, anti-human, REAfinity™

Overview

Clone REA758 recognizes the human FcεRIα, the α subunit of the human high-affinity Fc receptor for IgE. The receptor is composed of one α-, one β-, and two disulphide-linked γ-chains, of which the α subunit binds IgE1. FcεRIα is expressed on mast and basophil cells, is upregulated in the presence of IgE, and is found on a subset of blood dendritic cells. The FcεRI complex plays an important role in triggering IgE-mediated allergic reactions.
Additional information: Clone REA758 displays negligible binding to Fc receptors.

Alternative names

FCER1A, Fce1a, FcERI

Detailed product information

Technical specifications

CloneREA758
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenFcεRIα
Alternative names of antigenFCER1A, Fce1a, FcERI
Molecular mass of antigen [kDa]27
Distribution of antigenmast cells, basophils
Entrez Gene ID2205
RRIDAB_2651719, AB_2651720, AB_2651721, AB_2651722, AB_2651723, AB_2651724, AB_2651725, AB_2651726, AB_2651727, AB_2651728, AB_2651729, AB_2801889, AB_2651718

References for FcεRIα Antibody, anti-human, REAfinity™

Publications

  1. Hakimi, J. et al. (1990) The alpha subunit of the human IgE receptor (FcERI) is sufficient for high affinity IgE binding. J. Biol. Chem. 265: 22079-22081
  2. Hasegawa, S. et al. (1999) Functional expression of the high affinity receptor for IgE (FcepsilonRI) in human platelets and its`intracellular expression in human megakaryocytes. Blood 93: 2543-2551
  3. Takai, T. et al. (2000) Epitope analysis and primary structures of variable regions of anti-human FcepsilonRI monoclonal antibodies, and expression of the chimeric antibodies fused with human constant regions. Biosci. Biotechnol. Biochem. 64: 1856-1867
  4. Suzukawa, M. et al. (2005) IgE- and FcepsilonRI-mediated migration of human basophils. Int. Immunol. 17: 1249-1255
  5. Yokoi, H. et al. (2008) Inhibition of Fcepsilon RI-dependent mediator release and calcium flux from human mast cells by sialic acid-binding immunoglobulin-like lectin 8 engagement. J. Allergy Clin. Immunol. 121: 499-505
  6. Hammad, H. et al. (2010) Inflammatory dendritic cells–not basophils–are necessary and sufficient for induction of Tʜ2 immunity to inhaled house dust mite allergen. J. Exp. Med. 207: 2097-2111

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