Clone:
REA451
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC
Alternative names:
NOS3, ECNOS, NOS type III, EC-NOS, NOS III

Specifications for eNOS Antibody, anti-human, REAfinity™

Overview

Clone REA451 recognizes the human endothelial nitric oxide synthase (eNOS) antigen regardless of phosphorylation status. eNOS catalyzes the production of nitric oxide (NO) from l-arginine within the endothelium to regulate vascular tone via cGMP signaling in vascular smooth muscle, mitochondrial respiration, platelet function, inflammation, and angiogenesis. Physiologically, the most important stimulus for the continuous formation of NO is the shear stress generated by the streaming blood on the endothelial layer. Serine/threonine protein kinase Akt/PKB mediates the activation of eNOS, leading to increased NO production. Inhibition of the phosphatidylinositol-3-OH kinase/Akt pathway or mutation of the Akt site on eNOS protein attenuates the serine phosphorylation and prevents the activation of eNOS.
Additional information: Clone REA451 displays negligible binding to Fc receptors.

Alternative names

NOS3, ECNOS, NOS type III, EC-NOS, NOS III

Detailed product information

Technical specifications

CloneREA451
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, mouse
Cross-reactivitymouse
AntigeneNOS
Alternative names of antigenNOS3, ECNOS, NOS type III, EC-NOS, NOS III
Molecular mass of antigen [kDa]133
Distribution of antigenother
Entrez Gene ID4846
RRIDAB_2651630, AB_2651631, AB_2651628

References for eNOS Antibody, anti-human, REAfinity™

Publications

  1. Dimmeler, S. et al. (1999) Activation of nitric oxide synthase in endothelial cells by Akt-dependent phosphorylation. Nature 399(6736): 601-605
  2. King, A. L. et al. (2014) Hydrogen sulfide cytoprotective signaling is endothelial nitric oxide synthase-nitric oxide dependent. Proc. Natl. Acad. Sci. U.S.A. 111(8): 3182-3187
  3. Janssens, S. P. et al. (1992) Cloning and expression of a cDNA encoding human endothelium-derived relaxing factor/nitric oxide synthase. J. Biol. Chem. 267(21): 14519-14522

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