Clone:
REAL645
Type of antibody:
Releasable fluorochromes, Primary antibodies, Recombinant antibodies
Applications:
MICS, IHC, IF

Specifications for Cytokeratin HMW Antibody, anti-human, REAdye_lease™

Overview

Clone REAL645 is an antibody fragment derived from the full Cytokeratin HMW antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAdye_lease Complex to bind markers with high avidity.
Clone REAL645 recognizes the human Cytokeratin HMW (High Molecular Weight), which includes the cytokeratins 1,5,10 and 14. Keratins are polypeptides with keratin-containing intermediate filaments, which play a critical role in mechanical stability and integrity of epithelial cells and tissues, have regulatory functions and are involved in intracellular signaling pathways. Clone REAL645 detects squamous and ductal epithelium and other complex epithelia in normal tissues, e.g. basal cells in prostate, and myoepithelial cells in breast and corresponding tumors.
For removal of REAdye_lease fluorochromes for optional relabeling with different fluorochrome-conjugated REAdye_lease antibodies use the REAlease Support Kit (130-120-675).

Detailed product information

Technical specifications

CloneREAL645
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable fluorochromes, Primary antibodies, Recombinant antibodies
Specieshuman
AntigenCytokeratin HMW
Distribution of antigenepithelial cells, tumor cells
RRIDAB_2877037, AB_2877040

References for Cytokeratin HMW Antibody, anti-human, REAdye_lease™

Publications

  1. Moll, R. et al. (1982) The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31(1): 11-24
  2. Yang, X. J. et al. (1999) Rare expression of high-molecular-weight cytokeratin in adenocarcinoma of the prostate gland: a study of 100 cases of metastatic and locally advanced prostate cancer. Am. J. Surg. Pathol. 23(2): 147-152