The CRONUS laser is a novel, ultrafast laser expanding the possibilities of 2-photon microscopy. It provides two tunable (680–960 nm and 960–1300 nm) and one fixed (1025 nm) independent but synchronized femtosecond laser pulses. Its gap-free, broad tuning curves cover the entire excitation spectrum relevant for 2-photon microscopy applications. The CRONUS laser thus allows a free choice of fluorophores that can be excited simultaneously and moreover the detection of non-linear optical processes, such as second and third harmonic generation (SHG and THG).

Data and images for CRONUS Laser

Figures

Figure 1

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Dimensions of the CRONUS laser. From left to right: output A, B, and C.

Figure 1

Dimensions of the CRONUS laser. From left to right: output A, B, and C.

Figure 2

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CRONUS laser tuning curve for output A (black curve) and output B (teal curve).

Figure 2

CRONUS laser tuning curve for output A (black curve) and output B (teal curve).

Specifications for CRONUS Laser

Overview

The CRONUS laser is a novel, ultrafast laser expanding the possibilities of 2-photon microscopy. It provides two tunable (680–960 nm and 960–1300 nm) and one fixed (1025 nm) independent but synchronized femtosecond laser pulses. Its gap-free, broad tuning curves cover the entire excitation spectrum relevant for 2-photon microscopy applications. The CRONUS laser thus allows a free choice of fluorophores that can be excited simultaneously and moreover the detection of non-linear optical processes, such as second and third harmonic generation (SHG and THG).

Detailed product information

Background information

Thus far, the combination of a Ti:sapphire laser with an optical parametric oscillator (OPO) has been considered the gold standard for the simultaneous excitation of multiple fluorophores in 2-photon microscopy. However, this combination does not fully cover the excitation spectra of many fluorescent dyes and markers frequently used in life sciences. In contrast, the CRONUS laser provides a continuous excitation range between 680 and 1300 nm. Single or dual output broad band lasers, which are integrated into 2-photon microscopy systems nowadays, do not have the capability to excite multiple dyes at once. Integrated into our TriM Scope™ Multiphoton Microscopes, the CRONUS laser meets this challenge with its three synchronized output laser lines.
Benefits of the CRONUS laser for 2-photon microscopy in a nutshell:
  • free choice of fluorophores for 2-photon microscopy through two tunable (680–960 nm, 960–1300 nm) and one fixed (1025 nm) synchronized output channels
  • simultaneous excitation of fluorescent probes, calcium indicators, or opsins right at their spectral absorption maxima
  • compact and cost-efficient

Resources for CRONUS Laser