Clone:
REAL355
Type of antibody:
Releasable fluorochromes, Primary antibodies, Recombinant antibodies
Applications:
MICS, IHC, IF
Alternative names:
SLC3A2, SLC7A5, 4F2hc

Specifications for CD98 Antibody, anti-human, REAdye_lease™

Overview

Clone REAL355 is an antibody fragment derived from the full CD98 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAdye_lease Complex to bind markers with high avidity.
Clone REAL355 recognizes the human CD98 antigen, a single-pass type II membrane glycoprotein. CD98 is broadly expressed on non-hematopoietic cells and also on peripheral blood lymphocytes, monocytes, and granulocytes. The CD98 heterodimer consists of a 80–85 kDa heavy chain (CD98hc, also known as 4F2 antigen heavy chain or FRP-1) that is disulfide-linked with a multi-pass light chain of 40 kDa. The heavy chain binds to the cytoplasmic tails of integrin-β chains and mediates adhesive signals that control cell spreading, survival, and growth. The light chain functions in amino acid transport. Some of the light chains have broad specificity, but the large neutral amino acid transporters LAT-1 and LAT-2 have preference for importing certain essential amino acids, particularly leucine, isoleucine, and arginine (LAT-1), in exchange for glutamine. Through its nutrient function, CD98 can contribute to the survival and growth of many cell types. CD98hc functions in amplifying integrin signaling and in the transport of amino acids. Both of these functions can contribute to cell survival and proliferation.
For removal of REAdye_lease fluorochromes for optional relabeling with different fluorochrome-conjugated REAdye_lease antibodies use the REAlease Support Kit (130-120-675).

Alternative names

SLC3A2, SLC7A5, 4F2hc

Detailed product information

Technical specifications

CloneREAL355
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable fluorochromes, Primary antibodies, Recombinant antibodies
Specieshuman
AntigenCD98
Alternative names of antigenSLC3A2, SLC7A5, 4F2hc
Distribution of antigenlymphocytes, monocytes, granulocytes, kidney

References for CD98 Antibody, anti-human, REAdye_lease™

Publications

  1. Quackenbush, E. et al. (1987) Molecular cloning of complementary DNAs encoding the heavy chain of the human 4F2 cell-surface antigen: a type II membrane glycoprotein involved in normal and neoplastic cell growth. Proc. Natl. Acad. Sci. U.S.A. 84(18): 6526-6530
  2. Cantor, J. M. et al. (2012) CD98 at the crossroads of adaptive immunity and cancer. J. Cell. Sci. 125(6): 1373-1382
  3. Kanai, Y. et al. (1998) Expression cloning and characterization of a transporter for large neutral amino acids activated by the heavy chain of 4F2 antigen (CD98). J. Biol. Chem. 273(37): 23629-23632