Clone:
REAL508
Type of antibody:
Releasable antibodies, Primary antibodies, Recombinant antibodies
Applications:
FC
Alternative names:
PLAUR, U-PAR, URKR, uPAR, MO3

Extended validation for CD87 Antibody, anti-human,
REAlease
®

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REAL508
62022++
VIM5++
REA892++
Cells were incubated with an excess of purified unconjugated CD87 (REAL508) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD87. Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with CD87 antibodies and with a suitable counterstaining. As a control, CD87 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD87. Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with CD87 antibodies and with a suitable counterstaining. As a control, CD87 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD87. Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with CD87 antibodies and with a suitable counterstaining. As a control, CD87 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD87. Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with CD87 antibodies and with a suitable counterstaining. As a control, CD87 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD87 (REAL508). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD87 (REAL508). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD87 (REAL508). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD87 Antibody, anti-human,
REAlease
®

Overview

Clone REAL508 is an antibody fragment derived from the full CD87 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL508 recognizes the human CD87 antigen, a 36–38 kDa GPI-anchored cell-surface receptor. CD87, also known as uPAR, binds uPA and thus facilitating the catalytic C domain of the uPA to come in close proximity of membrane bound plasminogen. uPA is a serine protease, which converts plasminogen into plasmin which in-turn is involved in digestion of various extracellular matrix proteins. CD87 belongs to the Ly6/neurotoxin receptor family and consists of three disulfide-bonded homologous domains, D1, D2, and D3. Function of uPAR is modulated by two inhibitors, PA1 and PA2. The expression of membrane-bound CD87 is found on granulocytes, monocytes/macrophages, dendritic cells, fibroblasts, endothelial cells, and keratinocytes. Multiple known regulatory functions of CD87 include cell migration, leukocyte adhesion, chemotaxis, signal transduction, and tissue remodeling.
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

PLAUR, U-PAR, URKR, uPAR, MO3

Detailed product information

Technical specifications

CloneREAL508
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable antibodies, Primary antibodies, Recombinant antibodies
Specieshuman
AntigenCD87
Alternative names of antigenPLAUR, U-PAR, URKR, uPAR, MO3
Distribution of antigengranulocytes, monocytes, macrophages, dendritic cells, fibroblasts, endothelial cells, keratinocytes
RRIDAB_2857727, AB_2857732, AB_2857728, AB_2857730, AB_2857731

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