Clone:
MZ18-21F6
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1κ
Applications:
FC
Alternative names:
Ly9b, SLAMF5, hCD84, mCD84, GR6

Extended validation for CD84 Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with MZ18-21F6
CD84.1.21-
REA1102++
2G7++
Cells were incubated with an excess of purified unconjugated CD84 (MZ18-21F6) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for Anti-FCeRIa. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-FCeRIa antibodies and with a suitable counterstaining. As a control, Anti-FCeRIa antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-FCeRIa. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-FCeRIa antibodies and with a suitable counterstaining. As a control, Anti-FCeRIa antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-FCeRIa. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-FCeRIa antibodies and with a suitable counterstaining. As a control, Anti-FCeRIa antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-FCeRIa. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-FCeRIa antibodies and with a suitable counterstaining. As a control, Anti-FCeRIa antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for Anti-FCeRIa. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-FCeRIa antibodies and with a suitable counterstaining. As a control, Anti-FCeRIa antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD84 (MZ18-21F6). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD84 (MZ18-21F6). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD84 (MZ18-21F6). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD84 Antibody, anti-human

Overview

Human CD84 is a 65–80 kDa single-chain surface glycoprotein and member of the CD2 subset of the immunoglobulin (Ig) superfamily. CD84 is expressed on granulocytes, monocytes, macrophages, B cells, platelets, antigen-presenting cells, and subsets of T cells including thymocytes and mature T cells. Homophilic ligation of CD84 or ligation with CD84 antibodies has been shown to lead to enhanced proliferation of T cells as well as IFN-γ secretion. CD84 thus serves as a co-stimulatory molecule. CD84 gene expression has also been detected in lung and kidney tissue as well as on hematopoietic progenitor cells in bone marrow.

Alternative names

Ly9b, SLAMF5, hCD84, mCD84, GR6

Detailed product information

Technical specifications

CloneMZ18-21F6
Clonalitymonoclonal
Isotypemouse IgG1κ
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman
AntigenCD84
Alternative names of antigenLy9b, SLAMF5, hCD84, mCD84, GR6
Molecular mass of antigen [kDa]36
Distribution of antigenB cells, dendritic cells, granulocytes, macrophages, monocytes, NK cells, platelets, T cells, thymocytes
Entrez Gene ID8832
RRIDAB_1036232, AB_1036226, AB_1036228, AB_1036230

References for CD84 Antibody, anti-human

Publications

  1. Martin, M. et al. (2001) CD84 functions as a homophilic adhesion molecule and enhances IFN-γ secretion: adhesion is mediated by Ig-like domain 1. J. Immunol. 167: 3668-3676
  2. Tangye, S. G. et al. (2003) Functional requirements for interactions between CD84 and Src homology 2 domain-containing proteins and their contribution to human T cell activation. J. Immunol. 171: 2485-2495
  3. Zaiss, M. et al. (2003) CD84 expression on human hematopoietic progenitor cells. Exp. Hematol. 31: 798-805
  4. de la Fuente, M. A. et al. (1997) CD84 leukocyte antigen is a new member of the Ig superfamily. Blood 90: 2398-2405
  5. Romero, X. et al. (2004) Differential expression of SAP and EAT-2-binding leukocyte cell-surface molecules CD84, CD150 (SLAM), CD229 (Ly9) and CD244 (2B4). Tissue Antigens 64(2): 132-144

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