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Miltenyi Biotec distribution:
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
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Separation of CD8 + cells from PBMCs using CD8 MicroBeads, an LS Column, and a MidiMACS™ Separator. |
PBMCs before separation | CD8 - cells |
Figure 1Separation of CD8 + cells from PBMCs using CD8 MicroBeads, an LS Column, and a MidiMACS™ Separator. | Figure 1Separation of CD8 + cells from PBMCs using CD8 MicroBeads, an LS Column, and a MidiMACS™ Separator. |
CD8 + cells | |
Figure 1Separation of CD8 + cells from PBMCs using CD8 MicroBeads, an LS Column, and a MidiMACS™ Separator. |
Our lab routinely works with purified immune cell subsets from human peripheral blood mononuclear cells (PBMC). In order to obtain highly pure CD8+ T cells, as well as other immune cell types, we routinely employ magnetic separation with Miltenyi products for positive selection because of their ease of use and minimal optimization. We require highly pure CD8+ T cells due to the nature of our downstream analysis, and this product helps us achieve this goal.
The general aim of my research is to investigate the role of TCRs in the function of CTLs. For that we isolated TCR genes from T cell clones isolated from HIV patients and cloned them into lentiviral vectors to use for transduction of primary CD8 T cells isolated from naive patients. These transduced cells are then used for further study to evaluate their ability to recognize their cognate peptide and their killing capacity of infected cells. We selected this product because we had access to the AutoMACSpro, an automated machine used for cell separation compatible with these beads.
We are interested in enhancing the killing capacity of HIV-specific CD8 T cells by reengineering TCRs expressed by these cells. We use purified CD8 T cells from donors with known HLA types, to transduce them with the desired lentiviral construct and test their in vitro activity against HIV infected cells.
Great reagent to use for positive selection of CD8+ cells
Separation of CD8 + cells from PBMCs using CD8 MicroBeads, an LS Column, and a MidiMACS™ Separator. |
PBMCs before separation | CD8 - cells |
Figure 1Separation of CD8 + cells from PBMCs using CD8 MicroBeads, an LS Column, and a MidiMACS™ Separator. | Figure 1Separation of CD8 + cells from PBMCs using CD8 MicroBeads, an LS Column, and a MidiMACS™ Separator. |
CD8 + cells | |
Figure 1Separation of CD8 + cells from PBMCs using CD8 MicroBeads, an LS Column, and a MidiMACS™ Separator. |
Our lab routinely works with purified immune cell subsets from human peripheral blood mononuclear cells (PBMC). In order to obtain highly pure CD8+ T cells, as well as other immune cell types, we routinely employ magnetic separation with Miltenyi products for positive selection because of their ease of use and minimal optimization. We require highly pure CD8+ T cells due to the nature of our downstream analysis, and this product helps us achieve this goal.
The general aim of my research is to investigate the role of TCRs in the function of CTLs. For that we isolated TCR genes from T cell clones isolated from HIV patients and cloned them into lentiviral vectors to use for transduction of primary CD8 T cells isolated from naive patients. These transduced cells are then used for further study to evaluate their ability to recognize their cognate peptide and their killing capacity of infected cells. We selected this product because we had access to the AutoMACSpro, an automated machine used for cell separation compatible with these beads.
We are interested in enhancing the killing capacity of HIV-specific CD8 T cells by reengineering TCRs expressed by these cells. We use purified CD8 T cells from donors with known HLA types, to transduce them with the desired lentiviral construct and test their in vitro activity against HIV infected cells.
Great reagent to use for positive selection of CD8+ cells
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