Clone:
REA120
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
AGM6, B29, Igb

Extended validation for CD79b Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA120
CB3-1++
3A2-2E7++
Cells were incubated with an excess of purified unconjugated CD79b (REA120) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD79b. Human peripheral blood mononuclear cells (PBMCs) were stained with CD79b antibodies and with a suitable counterstaining. As a control, CD79b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD79b. Human peripheral blood mononuclear cells (PBMCs) were stained with CD79b antibodies and with a suitable counterstaining. As a control, CD79b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD79b. Human peripheral blood mononuclear cells (PBMCs) were stained with CD79b antibodies and with a suitable counterstaining. As a control, CD79b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD79b. Human peripheral blood mononuclear cells (PBMCs) were stained with CD79b antibodies and with a suitable counterstaining. As a control, CD79b antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD79b (REA120). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD79b (REA120). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD79b (REA120). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD79b Antibody, anti-human, REAfinity™

Overview

Clone REA120 detects the extracellular epitope of CD79b, the β chain of heterodimeric molecule CD79. CD79 is composed of an α chain (CD79a) and a β chain (CD79b) and is associated with surface immunoglobulins (Igs) to form the B cell receptor (BCR). CD79b is 37–39 kDa type I integral membrane protein and like CD79a contains an intracellular immunoreceptor tyrosine-based activation motif (ITAM). CD79a/CD79b heterodimer facilitates differentiation of pre–B cells from pro–B cells, surface expression of sIg, signal transduction following antigen recognition, and enodocytosis of recognised antigens. Patients with chronic lymphocytic leukemia (CLL) B cells lack a functional BCR which is often associated with mutations in CD79b coding region.
Additional information: Clone REA120 displays negligible binding to Fc receptors.

Alternative names

AGM6, B29, Igb

Detailed product information

Technical specifications

CloneREA120
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, non-human primate
Cross-reactivity
chimpanzee (
Pan troglodytes
)
AntigenCD79b
Alternative names of antigenAGM6, B29, Igb
Molecular mass of antigen [kDa]23
Distribution of antigenB cells
Entrez Gene ID974
RRIDAB_2734072, AB_2857653, AB_2857626, AB_2659214, AB_2659215, AB_2659216, AB_2659217, AB_2659218, AB_2659219, AB_2734071

References for CD79b Antibody, anti-human, REAfinity™

Publications

  1. Lin, J. and Justement, L. B. (1992) The MB-1/B29 heterodimer couples the B cell antigen receptor to multiple src family protein tyrosine kinases. J. Immunol. 149: 1548-1555
  2. Koyama, M. et al. (1997) CD79α/CD79β heterodimers are expressed on pro-B cell surfaces without associated μ heavy chain. Int. Immunol. 9: 1767-1772
  3. Gordon, M. S. et al. (2000) Aberrant B cell receptor signaling from B29 (Igβ, CD79b) gene mutations of chronic lymphocytic leukemia B cells. Proc. Natl. Acad. Sci. U.S.A. 97: 5504-5509

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