Clone:
REA1168
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC, MICS, IF, IHC
Alternative names:
Igα, MB-1

Extended validation for CD79a Antibody, anti-human, REAfinity™

Specificity

Other clonesOverlap in epitope recognition with REA1168
HM47++
Cells were incubated with an excess of purified unconjugated CD79a (REA1168) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for CD79a Antibody, anti-human, REAfinity™

Overview

Clone REA1168 regognizes the cytoplamic domain of CD79a, which is also known as MB-1 and Igα. Along with CD79b (Igβ), CD79a is associated with surface Ig (sIg) to form the B cell antigen receptor complex. CD79a is a 47 kDa glycoprotein and comprises a single extracellular immunoglobulin domain, a transmembrane domain, and a signaling intracellular domain with immunoreceptor tyrosine-based activation motif (ITAM). CD79a/CD79b heterodimer facilitates differentiation of pre–B cells from pro–B cells, surface expression of sIg, signal transduction following antigen recognition, and enodocytosis of recognised antigens. Surface expression of CD79a dimishes in plasma cells, where it is mainly found as an intracellular molecule. CD79 is considered a B cell–specific marker and is often used to identify the B cell lineage of acute lymphoblastic leukemia. Recent reports however suggest expression of CD79a in T cell acute lymphoblastic leukemia. Individuals lacking CD79a have low/absent circulating pre–B cells and serum antibodies due to a block in B cell development.
Additional information: Clone REA1168 displays negligible binding to Fc receptors.

Alternative names

Igα, MB-1

Detailed product information

Technical specifications

CloneREA1168
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, non-human primate, mouse
Cross-reactivity
chimpanzee (
Pan troglodytes
)
,
olive baboon (
Papio anubis
)
, mouse
AntigenCD79a
Alternative names of antigenIgα, MB-1
Molecular mass of antigen [kDa]22
Distribution of antigenplasma cells, plasma B cells, leukemia cells
Entrez Gene ID973
RRIDAB_2784279, AB_2784278, AB_2784277, AB_2784282, AB_2784281, AB_2784284, AB_2784283, AB_2784280

References for CD79a Antibody, anti-human, REAfinity™

Publications

  1. Mason, D. Y. et al. (1991) The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells. J. Immunol. 147(11): 2474-2482
  2. Lin, J. and Justement, L. B. (1992) The MB-1/B29 heterodimer couples the B cell antigen receptor to multiple src family protein tyrosine kinases. J. Immunol. 149: 1548-1555
  3. Koyama, M. et al. (1997) CD79α/CD79β heterodimers are expressed on pro-B cell surfaces without associated μ heavy chain. Int. Immunol. 9: 1767-1772
  4. Minegishi, Y. et al. (1999) Mutations in Igalpha (CD79a) result in a complete block in B-cell development. J. Clin. Invest. 104(8): 1115-1121
  5. Lai, R. et al. (2000) Flow cytometric detection of CD79a expression in T-cell acute lymphoblastic leukemias. Am. J. Clin. Pathol. 113(6): 823-830

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