Clone:
REAL328
Type of antibody:
Releasable antibodies, Primary antibodies, Recombinant antibodies
Applications:
FC, MICS, IHC, IF
Alternative names:
ITGB3, BDPLT16, BDPLT2, GP3A, GPIIIa, GT

Extended validation for CD61 Antibody, anti-human,
REAlease
®

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA761
VI-PL2++
RUU-PL7F12++
PM6/13++
Y2/51++
REA761++
Cells were incubated with an excess of purified unconjugated CD61 (REAL328) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for CD61 Antibody, anti-human,
REAlease
®

Overview

Clone REAL328 is an antibody fragment derived from the full CD61 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL328 recognizes the human CD61 antigen, a cell surface protein belonging to the integrin family. CD61 is also known as the integrin β3-subunit. It associates with CD41 to form the heterodimeric gpIIb/gpIIIa complex that is present on human megakaryocytes and platelets and mediates cell adhesion processes. Together with CD51, CD61 forms the vitronectin receptor, which is present on platelets as well as on a variety of other cell types such as osteoclasts and endothelial vessel cells. Clone REAL328 specifically recognizes the integrin β3-chain of both heterodimeric complexes.
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

ITGB3, BDPLT16, BDPLT2, GP3A, GPIIIa, GT

Detailed product information

Technical specifications

CloneREAL328
Clonalitymonoclonal
Isotype controlControl Antibody
Hostcell line
Type of antibodyReleasable antibodies, Primary antibodies, Recombinant antibodies
Specieshuman
AntigenCD61
Alternative names of antigenITGB3, BDPLT16, BDPLT2, GP3A, GPIIIa, GT
Distribution of antigenmegakaryocytes, platelets, other
RRIDAB_2819690, AB_2819694