CD56 MicroBeads were developed for the positive selection or depletion of natural killer (NK) cells from human PBMCs or single-cell suspensions from tissues. The CD56 antigen is expressed by most NK cells and a minor T cell subset (CD3
+
CD56
+
NKT cells). Upon activation of NK cells, the surface expression of CD56 is increased.

Data and images for CD56 MicroBeads, human

Figures

Figure 1

CD56
+
cells were isolated from human peripheral blood mononuclear cells (PBMCs) using CD56 MicroBeads, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD56-PE and CD3-APC and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
PBMCs before separation
Isolated CD56
+
cells
View details

Figure 1

CD56
+
cells were isolated from human peripheral blood mononuclear cells (PBMCs) using CD56 MicroBeads, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD56-PE and CD3-APC and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

CD56
+
cells were isolated from human peripheral blood mononuclear cells (PBMCs) using CD56 MicroBeads, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD56-PE and CD3-APC and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD56 MicroBeads, human

Overview

CD56 MicroBeads were developed for the positive selection or depletion of natural killer (NK) cells from human PBMCs or single-cell suspensions from tissues. The CD56 antigen is expressed by most NK cells and a minor T cell subset (CD3
+
CD56
+
NKT cells). Upon activation of NK cells, the surface expression of CD56 is increased.

Detailed product information

Downstream applications

CD56
+
cells are used in studies on NK cell development, proliferation, or cytotoxic activity as well as in analysis of signal transduction and related molecular processes
1–3
.
CD56 may also be used for the enrichment of embryonic stem cell–derived cardiomyocytes cells after
in vitro
differentiation
4
as well as the direct selection stem and progenitor cells from muscle tissue
5,6
.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.

Reviews for CD56 MicroBeads, human

NK Cell Separation Beads

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CD56 MicroBeads, human (130-050-401)

Our laboratory investigates different ways to improve immune responses against HIV, including enhancement of the capacity of effector cells, such as NK cells, to kill HIV-infected cell. We are currently investigating the capacity of an engineered antibody to trigger NK-cell mediated ADCC activity in in vitro assays against an HIV-infected cell line. We use purified human NK cells from donors to use as effector cells in these assays.

CD56 Microbeads For Purification Of Human NK Cells

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CD56 MicroBeads, human (130-050-401)

Our laboratory investigates different ways to improve immune responses against HIV, including NK mediated ADCC. For our in vitro and in vivo ADCC assays we need to isolate or deplete NK cells from human PBMCs, for which we use these beads.

CD56 Magnetic Beads

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CD56 MicroBeads, human (130-050-401)

Isolation of NK cells is of importance to several projects in our lab. These beads have a very easy protocol for isolation of CD56+ cells.

References for CD56 MicroBeads, human

Publications

  1. Fletcher, J. M. et al. (1998) Natural killer cell lysis of cytomegalovirus (CMV)-infected cells correlates with virally induced changes in cell surface lymphocyte function-associated antigen-3 (LFA-3) expression and not with the CMV-induced down-regulation of cell surface class I HLA. J. Immunol. 161: 2365-2374
  2. Doherty, D. G. et al. (1999)
    The human liver contains multiple populations of NK cells, T cells, and CD3
    +
    CD56
    +
    natural T cells with distinct cytotoxic activities and Tʜ1, Tʜ2, and Tʜ0 cytokine secretion patterns.
    J. Immunol. 163: 2314-2321
  3. Kumaki, S. et al. (2001) Identification of anti-herpes simplex virus antibody-producing B cells in a patient with an atypical RAG1 immunodeficiency. Blood 98: 1464-1468
  4. Xu, S. et al. (2006) Cardiac bodies: a novel culture method for enrichment of cardiomyocytes derived from human embryonic stem cells. Stem Cells Dev. 15: 631-639
  5. Sinanan, A. et al. (2004) Human adult craniofacial muscle-derived cells: neural-cell adhesion-molecule (NCAM; CD56)-expressing cells appear to contain multipotential stem cells. Biotechnol. Appl. Biochem. 40: 25-34
  6. De Luna, N. et al. (2006)
    Absence of dysferlin alters myogenin expression and delays human muscle differentiation
    in vitro
    .
    J. Biol. Chem. 281: 17092-17098

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