Clone:
JS11
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1κ
Applications:
FC, MICS, IF, IHC
Alternative names:
CR, CROM, DAF, TC

Extended validation for CD55 (DAF) Antibody, anti-human

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
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Fluorescence microscopy image of CD55 (DAF) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD55 (DAF)-PE (JS11, red) and counterstained with DRAQ5 (blue) as DNA stain.
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Fluorescence microscopy image of CD55 (DAF) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD55 (DAF)-PE (JS11, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD55 (DAF) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD55 (DAF)-PE (JS11, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of CD55 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD55-PE, clone (F1 JS11). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD55 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD55-PE, clone (F1 JS11). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD55 (DAF) Antibody, anti-human

Overview

Clone JS11 recognizes the CD55 antigen, a 70 kDa glycosylphosphatidylinositol-anchored single chain glycoprotein, which is also known as complement decay-accelerating factor (DAF). CD55 is broadly distributed among hematopoietic and non-hematopoietic cells. It is expressed on the plasma membranes of all cell types that are in intimate contact with serum and on the surfaces of epithelial cells lining the extracellular compartments. Variants of the molecule are present in body fluids and in extracellular matrix. CD55 plays multiple physiologic roles including tissue protection from the cytotoxic complement injury, anti-inflammatory function owing to its anti-adherence properties which enhance transmigration of monocytes and macrophages and reduce tissue injury. CD55 plays an essential role during pregnancy and is involved in the protection of the semiallogeneic fetus and in preventing uncontrolled infiltration by white cells. It is exploited in multiple pathologic conditions by pathogens and viruses in chronic tissue infection processes.

Alternative names

CR, CROM, DAF, TC

Detailed product information

Technical specifications

CloneJS11
Clonalitymonoclonal
Isotypemouse IgG1κ
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman
AntigenCD55 (DAF)
Alternative names of antigenCR, CROM, DAF, TC
Distribution of antigenB cells, epithelial cells, monocytes, NK cells, T cells
RRIDAB_2658718, AB_2658719, AB_2658720, AB_2658721, AB_2658722, AB_2658723, AB_2658724, AB_2658725, AB_2658726, AB_2658727

References for CD55 (DAF) Antibody, anti-human

Publications

  1. Caras, I. W. et al. (1987) Cloning of decay-accelerating factor suggests novel use of splicing to generate two proteins. Nature 325(6104): 545-549
  2. Nowicki, B. et al. (2013) DAF as a therapeutic target for steroid hormones: implications for host-pathogen interactions. Adv. Exp. Med. Biol. 734: 83-96
  3. Osuka, F. et al. (2006) Molecular cloning and characterization of novel splicing variants of human decay-accelerating factor. Genomics 88(3): 316-322

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