Clone:
REA541
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
Itga2, gpIa, VLA-2α, VLA-2alpha, DX5

Extended validation for CD49b Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA541
DX5++
HMα2-
REA981++
Cells were incubated with an excess of purified unconjugated CD49b (REA541) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for CD49b Antibody, anti-mouse, REAfinity™

Overview

Clone REA541 recognizes the mouse CD49b antigen, a single-pass type I membrane protein also known as integrin alpha-2 (ITGA2). CD49b together with CD29 (ITGB1) forms the adhesion molecule very late antigen-2 (VLA-2), which binds mainly to collagens I, II, and XI. VLA-2 is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation, and organization of newly synthesized extracellular matrix. It is also a receptor for laminins, collagen C-propeptides, and E-cadherin. Mice homozygous for a null mutation in the CD49b gene die very early in embryogenesis. CD49b is expressed on the vast majority of mouse NK cells and on a subset of NKT cells. It is not as mouse strain-restricted as other NK cell markers, for example, NK1.1, and is expressed by most common inbred mouse strains. Bone marrow (BM) resident memory T helper (Tʜ) cells also express CD49b and the homing of adoptively transferred BM memory Tʜ cells to BM can be efficiently blocked by antibodies to CD49b. This points to a decisive role of CD49b in the trafficking of memory Th cell precursors to the BM niches for memory Tʜ cells.
Additional information: Clone REA541 displays negligible binding to Fc receptors.

Alternative names

Itga2, gpIa, VLA-2α, VLA-2alpha, DX5

Detailed product information

Technical specifications

CloneREA541
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD49b
Alternative names of antigenItga2, gpIa, VLA-2α, VLA-2alpha, DX5
Molecular mass of antigen [kDa]126
Distribution of antigenNK cells, NKT cells, T helper cells
Entrez Gene ID16398
RRIDAB_2752210, AB_2784229, AB_2784228, AB_2784230, AB_2658473, AB_2658474, AB_2658477, AB_2658478, AB_2752212

References for CD49b Antibody, anti-mouse, REAfinity™

Publications

  1. Gagliani, N. et al. (2013) Coexpression of CD49b and LAG-3 identifies human and mouse T regulatory type 1 cells. Nat Med 19(6): 739-746
  2. Hanazawa, A. et al. (2013) CD49b/CD69-dependent generation of resting T helper cell memory. Front Immunol 4: 183
  3. Edelman, J. M. et al. (1994) The mouse VLA-2 homologue supports collagen and laminin adhesion but not virus binding. Cell Adhes. Commun. 2(2): 131-143

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